摘要
目的探讨用快速试验方法检测A群溶血性链球菌抗原在临床上的应用以及和细菌培养鉴定法的比较。方法检测A链抗原的快速试验方法采用免疫层析试验(Immunochromatographic Assay,I-CA)原理。采集2005年至2006年来我院就诊的上呼吸道感染病人咽拭子911份作快速A链抗原检测,并从中随机选取214个病人,另外再采集一份标本同时做常规培养,分析快速法的敏感性和特异性,比较两种方法的优缺点。结果在上呼吸道感染病人中,A群溶血性链球菌感染率为10.1%,在细菌引起的上感中感染率最高。快速A链抗原检测法的敏感性为82.1%,特异性为98.9%。结论快速A链抗原检测法的敏感性和特异性都比较高,是诊断A群溶血性链球菌感染的一个新的选择方案,可以帮助医生在最早时间内作出诊断和选择治疗方案,有效降低抗生素使用率,适合向临床推广。
Objective To evaluate the application and advantages of rapid antigen detection in diagnosis of group A β-haemolytic streptococcal upper respiratory tract infection. Methods The principle of rapid group A streptococcal antigen detection was applied by immonochromatographic assay. We collected 911 throat swabs from patients with upper respiratory tract infection from year 2005 to 2006 to perform rapid test for group A β-haemolytic streptococci, and we performed both rapid test and culture for 214 patients of them. We compared the two methods and assessed the diagnostic value of rapid antigen detection for group A streptococcal infection. Results Group A β-haemolytic streptococci was the most common cause of bacterial upper respiratory tract infection with the rate of 10.1% in all upper respiratory tract infection patients. The rapid antigen detection had a sensitivity of 82.1% and specificity of 98.9%. Conclusions Rapid antigen detection for group A streptococcal infection has a high sensitivity and specificity. It allows doctors to make diagnosis fast and initiate appropriate therapy immediately. It proves to be a new reliable and practical method for detection of group A β-haemolytic streptococcal infection with many advantages, especially tremendous minimization of unnecessary antibiotic treatment.
出处
《江西医学检验》
2007年第5期419-422,共4页
Jiangxi Journal of Medical Laboratory Sciences
关键词
A群溶血性链球菌
快速抗原检测法
免疫层析试验
Group A β-haemolytic streptococcus
Rapid antigen detection
Immonochromatographic assay
