摘要
采用免疫组织化学方法分析食管癌组织和癌旁组织中肿瘤抑制基因P16的变化,并与肿瘤抑制基因P53蛋白聚集的变化进行比较研究。结果:22例食管鳞癌组织中,3例出现P16蛋白的免疫阳性反应(14%),此3例均为高分化鳞癌。在3例P16免疫阳性组织中,2例同时出现P53蛋白聚集的改变。22例手术切除癌标本中的各级癌前病变均未出现P16阳性反应。癌组织和癌旁上皮各级病变均出现较高的P53蛋白改变。提示:肿瘤抑制基因P16的变化可能主要发生在食管癌变的终末阶段,P16与P53有较高的一致性改变。
The changes of tumor suppressor gene P16 and its correlation with tumor suppressor gene P53 protein accumulation were determined in esophageal cancer and its adjacent cancer tissues with immunohistochemical method.The results demonstrated that,of the 22 primary esophageal squamous cell carcinomas,3 showed positive immunoreactivity of P16 (14%).All the 3 cases were highly differentiated.Of the 3 cases with P16 positive immunostaining,2 cases showed P53 protein accumulation.The precancerous lesions with different degrees from 22 surgically resected esophageal specimens did not show P16 protein expression.On the other hand,P53 protein accumulation was found in cancer and precancerous lesions from the surgically resected esophageal cancer specimens.The present results indicated that the changes of tumor suppressor gene P16 may occur at the final stage of esophageal carcinogenesis.There was a high co incidence for P16 and P53 changes.
出处
《河南医科大学学报》
1997年第1期34-36,共3页
Journal of Henan Medical University
基金
卫生部优秀青年科技人才专项科研基金
河南省首批杰出青年科学基金
中美合作课题