摘要
目的:建立高同型半胱氨酸血症动物模型,观察其血浆一氧化氮和内皮素1浓度的变化,验证对血管内皮功能的损伤作用。方法:实验于2006-10在新疆医科大学实验动物中心完成。①实验动物:雄性6～8周龄Wistar大鼠30只,随机数字表法分为普通饲料组、高蛋氨酸组,15只/组。②实验方法:高蛋氨酸组以普通饲料+3%蛋氨酸喂养1个月,建立高同型半胱氨酸动物模型,大鼠血浆同型半胱氨酸浓度高于12.94μmol/L代表造模成功。正常对照组以普通饲料喂养1个月。③实验评估:造模前及造模后30d,两组均清晨空腹采血,应用酶联免疫吸附法测定血浆同型半胱氨酸浓度,硝酸还原酶法测定血浆一氧化氮浓度,放射免疫法测内皮素1浓度。结果:30只大鼠均进入结果分析。①造模前后血浆同型半胱氨酸浓度的变化:造模前两组血浆同型半胱氨酸浓度基本相似(t=0.133,P=0.895);造模后30d高蛋氨酸组明显高于正常对照组(t=25.291,P=0.000)。②造模前后血浆一氧化氮浓度的变化:造模前两组血浆一氧化氮浓度基本相似(t=0.142,P=0.888);造模后30d高蛋氨酸组明显低于正常对照组(t=2.121,P=0.043)。③造模前后血浆内皮素1浓度的变化:造模前两组血浆内皮素1浓度基本相似(t=0.894,P=0.378);造模后30d高蛋氨酸组明显高于正常对照组(t=4.937,P=0.000)。结论:当存在高同型半胱氨酸血症时,血浆一氧化氮水平降低,内皮素1水平升高,两者的动态平衡被破坏,证实血管内皮功能受损。
AIM：To establish animal model of hyperhomocysteinemia, so as to confirm that hyperhomocysteinemia could damage endothelial function by observing the changes of plasma nitric oxide （NO） and endothelin-1 （ET-1）. METHODS： The experiment was conducted in the Animal Research Center of Xinjiang Medical University in October 2006. （1）Thirty 6-8 week-old male Wistar rats were randomly divided into Methionine （Met） group and control group with 15 rats in each group. （2）The rats in Met group were fed with a standard rat diet supplemented with 3% Met for 1 month to establish animal model of hyperhomocysteinemia. Hyperhomocysteinemia was defined as the level of homocysteine in plasma exceeded 12.94 μmol/L. Control group was fed with a standard rat diet for 1 month. （3）Blood samples of all rats were taken before and 30 days after modeling. The levels of total plasma homocysteine were measured using enzymoimmunoassay. The levels of plasma homocysteine were measured by ELISA, NO with using nitrate reductase test, and levels of plasma ET-1 with radioimmunoassay. RESULTS： Thirty rats were involved in the result analysis. （1）The plasma homocysteine levels were similar between two groups before modeling （t =0.133, P =0.895）, but that in Met group was obviously higher than control group 30 days after modeling （t =25.291, P =0.000）. （2）The plasma NO levels were similar between two groups before modeling （t = 0.142, P =0.888）, but 30 days after modeling, Met group was obviously lower than control group （t =2.121, P =0.043）. （3） The plasma ET-1 levels were similar between two groups before modeling （t =0.894,P =0.378）, while Met group was obviously higher than control group 30 days after modeling （t =4.937, P =0.000）. CONCLUSION： When hyperhomocysteinemia happens, plasma nitric oxide levels are reduced, plasma ET-1 levels increased, and the balance between them destructed, indicating endothelial function is impaired.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第41期8242-8246,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
