摘要
目的:建立不经衍生用高效液相色谱-蒸发光散射检测法直接测定复方氨基酸注射液(3AA)中 Val、Iso、Leu 的含量测定方法。方法:色谱柱为 Phenomenex Luna C_(18)(4.6mm×250mm,5μm);流动相为0.3%三氯乙酸溶液-乙腈(90:10),流速0.6mL·min^(-1),EISD 漂移管温度109℃,载气流速3.0 L·min^(-1)。结果:Val、Iso、Leu 的线性范围分别为1.008～5.040mg·mL^(-1)(r=0.9992),1.082～5.410mg·mL^(-1)(r=0.9992),1.343～6.714mg·mL^(-1)(r=0.9993);回收率分别为98.7%～100.9%(RSD<0.3%),98.5%～100.7%(RSD<0.2%),98.5%～101.0%(RSD<0.2%)。结论:本法简便快速,准确可靠,适用于复方氨基酸注射液(3AA)中3种组分的同时测定。
Objective：To establish a method for the determination of Val, Iso and Leu in compound amino acid in- jection（3AA） using HPLC with evaporative light- scattering detector （ELSD） without derivatization. Method： Chromatography was proformed on a Phenomenex Luna C18 column（4. 6 mm × 250 mm ,5 μm）. The mobile phase was composed of 0. 3% trifluoroacetic acid -acetonitrile （90：10 ） with the flow rate of 0.6 mL · min^-1. The gas flow rate was 3.0 L · min^-1 and the tube temperature was 109 ℃. Result. A satisfactory separation among three components was obtained. The linear response ranges were ： 1. 008 - 5. 040 mg · mL^-1 for Val （ r = 0. 9992） , 1. 082 -5.410 mg · mL^-1 for Iso（r =0. 9992） and 1. 343-6. 714 mg · mL^-1 for Leu（r =0. 9993） respectively. The recoveries of Val,Iso and Leu were 98.7% - 100. 9% （RSD 〈0. 3% ） ,98. 5% - 100. 7% （RSD 〈0. 2% ） ,98.5% -101.0% （RSD 〈0. 2% ） respectively. Conclusion：This method is simple, quick and the result of determination is precise and accurate. This method is suitable for determination of the three Components in compound amino acid injection（3AA）.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第10期1649-1651,共3页
Chinese Journal of Pharmaceutical Analysis
