摘要
目的:建立高效液相色谱法测定醋酸曲安奈德尿素乳膏中醋酸曲安奈德的含量。方法:采用 Shim-pack C_(18)色谱柱(4.6mm×250mm,5μm);以甲醇-水(75:25)为流动相;流速1.0mL·min^(-1);检测波长为240nm;柱温:室温。结果:醋酸曲安奈德线性范围为15.23～35.55μg·mL^(-1)(r=0.9999),平均回收率为99.8%,RSD 为0.77%(n=6)。结论:本方法简便、灵敏、准确,能有效地控制醋酸曲安奈德尿素乳膏的质量。
Objective：To establish a method for determination of triamcinolone acetonide acetate in triamcinolone and urea cream by HPLC. Methods：The chromatographic column of Shim -pack C18 （4. 6 mm×250 ram, 5 μm） was used, the mobile phase was methanol - water （ 75：25 ） with 1.0 mL · mm^-1 of flow rate, the detection wave- length was set at 240 nm and the chromatography was operated at room temperature. Results：The linear range was 15. 23 -35. 55 μg · mL^-1 （r =0. 9999） ,the average recovery was 99. 8% ,RSD was 0. 77% （n =6）. Conclusion： The method is convenient, accurate and suitable for determination of triamcinolone acetonide acetate in Triamcinolo- ne and Urea Cream.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第10期1652-1653,共2页
Chinese Journal of Pharmaceutical Analysis
