摘要
目的探讨益肾活血提取液(YSHXC)对同型半胱氨酸(HCY)诱导的兔主动脉平滑肌细胞(VSMCs)增殖的影响。方法组织贴块法体外原代培养兔VSMCs,建立HCY诱导的VSMCs增殖模型并分组:正常组、HCY刺激组、HCY+YSHXC组、HCY+叶酸组。采用MTT法检测细胞的增殖活度,免疫组化技术检测增殖细胞核抗原(PCNA)的表达,并采用流式细胞术检测细胞周期。结果YSHXC及叶酸均能降低PC-NA的表达(P<0.01),且中药组低于叶酸组(P<0.05)。细胞周期也有相应的变化,与HCY组相比,YSHXC组G0/G1期细胞比例显著升高,S期与G2/M期细胞显著降低(P<0.01),基本接近正常对照组(P>0.05)。结论YSHXC能抑制HCY诱导的VSMCs增殖,下调细胞内PCNA的表达,这可能与YSHXC推动细胞周期时相的改变有关。
Objeetive To explore the effects of the Yishenhuoxue Capsule（YSHXC） on the proliferation of vascular smooth muscle cells （VSMCs） induced by HCY in rabbits. Methods VSMCs were cultured by tissue explants in vitro and proliferation was induced by HCY. Cells were divided into four groups： the control group, the HCY group, the Yishenhuoxue group and the folic acid group. The proliferation of cells, the expression of PCNA and cell cycles were determined by MTT, immunohistochemistry and flow cytometric technique. Results After being separately incubated with Yishenhuoxue and folic acid, the expression of PCNA was effectively reduced（ P 〈 0.01 ） and that was especially in the Yishenhuoxue group（ P 〈 0.05）. Cell cycles were also homologically changed. Compared with the HCY group, the cell number of the Yishenhuoxue group in the G0/G1 period was increased while in the S period was decreased, which showed that the cell number in the synthesis period of DNA went down remarkably（ P 〈 0.01 ）. Conclusion Yishenhuoxue can significantly inhibit the proliferation of ceils induced by HCY and bring down the level of PCNA, which may be related to a change of the cell cycle. Therefore, the Yishenhuoxue Capsule can delay the process of atheroscleorsis at an earlier period.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第10期1011-1015,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金资助课题(30572452)
关键词
益肾活血提取液
血管平滑肌细胞
增殖
细胞周期
增殖细胞核抗原
Yishenhuoxue Capsule
Vascular smooth muscle cells
Proliferation
Cell cycle
Proliferating cell nu clear antigen
