摘要
目的:降低移植神经的抗原性、抑制异体神经移植中的排斥反应是目前的研究焦点。观察小鼠胸腺内注射异基因抗原对异体鼠坐骨神经移植存活的干预作用。方法:实验于2006-06/2007-05在哈尔滨医科大学附属第四医院实验室完成,实验方法符合动物伦理学要求。①选用雄性C57BL/6(H-2b)供体鼠32只,雌性Balb/c(H-2d)受体鼠44只,将坐骨神经移植受体鼠按随机数字表法分为4组(n=11):主要组织相容性复合物(H-2b)抗原注射组自供体小鼠的脾细胞中提取抗原注入受体鼠胸腺内,于2周后移植供体鼠坐骨神经;免疫抑制剂组移植前3d开始腹腔注射环孢霉素A;异体神经移植组、自体神经移植组单纯进行异体及自体神经移植。②于神经移植3周后进行电生理学、组织学及免疫学检测。结果:44只受体鼠全部进入结果分析。①坐骨神经移植段的神经传导速度:异体神经移植组显著小于主要组织相容性复合物(H-2b)抗原注射组(P<0.05),自体神经移植组显著大于主要组织相容性复合物(H-2b)抗原注射组(P<0.05),主要组织相容性复合物(H-2b)抗原注射组与免疫抑制剂组差异无显著性意义(P>0.05)。②组织学检查证明自体神经移植组及主要组织相容性复合物(H-2b)抗原注射组均有大量神经纤维再生,并已通过移植体。③混合淋巴细胞反应及迟发型超敏反应结果:主要组织相容性复合物(H-2b)抗原注射组对供体小鼠淋巴细胞反应呈特异性减弱,与异体神经移植组及免疫抑制剂组比较其差异具有显著性意义(P<0.05)。结论:胸腺内注射异基因主要组织相容性复合物抗原可诱导对异体坐骨神经移植的特异性免疫耐受,神经恢复效果优于单纯异体神经移植,等同于免疫抑制剂使用组。
AIM： Current studies emphasize on inhibiting the immune rejection in nerve allograft and reducing the antigenicity of nerve. This study is designed to investigate the effect of intrathymic injection of allogene on the survival of sciatic nerve allograft in mice. METHODS： The experiment was carried out in the 4^th Affiliated Hospital of Harbin Medical University from June 2006 to May 2007. The experimental procedure was consistent with the ethics requirement of animals. （1）Thirty-two male C57BL/6（H-2°） mice were used as donors. Forty-four female Balb/c（H-2°） mice, used as recipients, were randomly divided into 4 groups （n =11）. Group A： The major histocompatibility complex （MHC, H-2°） antigen was extracted from spleen cells of C57BL/6 （H-2°） donor mice and then injected into the thymus of Balb/c （H-2°） recipient mice. Then allotransplantation of the sciatic nerve from donor to recipient mice was done 2 weeks later; group B was intraperitoneally injected with immunosuppressant （cyclosporin A） from the third day before operation; group C progressed nerve allograft; group D progressed autologous nerve graft. （2） Electrephysiological, histological, and immunological assays of four groups were carried out 3 weeks after the transplantation. RESULTS： All 44 recipient mice were involved in the result analysis. （1）The motor nerve conduction velocity in group A was significantly higher than that of group C （P 〈 0.05）, but significantly lower than that of group D （P 〈 0.05）. There was no significant difference compared with group B （P 〉 0.05）.（2）Histological examination evidenced that, a large amount of nerve fiber regenerated through the graft in groups A and D. （3） Mixed lymphocyte reaction and delayed type hypersensitivity showed that, the lymphocyte reaction in the donor mice of group A decreased in a specific way, which was significantly different from groups B and C （P 〈 0.05）. CONCLUSION： The intrathymic injection of allogene MHC antigen may induce specific immunologic tolerance to sciatic nerve allografts in mice. The nerve recovery is identical with that of immunosuppressant use group and superior to that of simple nerve allograft group.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第43期8657-8660,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
黑龙江省教育厅科学技术研究项目~~
