摘要
建立了一种采用Shim-pack C18-ODS柱,以乙腈-0.05%磷酸溶液（13∶87）为流动相,检测波长为290 nm,同时测定胡黄连中三种苷元有机酸的反相高效液相色谱方法。香草酸、阿魏酸和肉桂酸的线性范围分别为14.0-210.0 ng（R=0.9996）、52.0-520.0 ng（R=0.9995）和6.0-90.0 ng（R=0.9993）,平均回收率分别为96.6%、98.9%和97.4%。
The simultaneous determination of vanillic acid, ferulic acid and cinnamic acid in Picrohiza scrophulariiflora Pennell by RP-HPLC method was reported in this paper. The HPLC separation was performed on a Shim-pack C18-ODS column（250 mm×4.6 mm,5 μm） with mobile phase of acetonitrile-0.05% phosphoric acid （13 ： 87） and detected at wavelength 290 nm. The calibration curve was linear in the range of 14.0-210.0 ng for vanillic acid （R = 0. 9996）, 52.0 - 520.0 ng for ferulic acid （R = 0. 9995） and 6.0 - 90.0 ng for cinnamic acid（R=0. 9993）. The average recovery of vanillic acid,ferulic acid and cinnamic acid was 96.6%, 98. 9% and 97.4% ,respectively. The method was accurate with a good reproducibility.
出处
《化学与生物工程》
CAS
2007年第10期75-76,共2页
Chemistry & Bioengineering
关键词
高效液相色谱
胡黄连
香草酸
阿魏酸
肉桂酸
H PLC
Picrohiza scrophula riiflora Pennell
vanillic acid
ferulic acid
cinnamic acid
