摘要
目的:探讨非小细胞肺癌(non-small-cell lung cancer,NSCLC)区域淋巴结人肺组织特异性基因(lunx mRNA)的表达与预后的关系。方法:应用SYBR GreenⅠ嵌合荧光法进行逆转录聚合酶链反应(real-timeRT-PCR)用以检测74例NSCLC手术切除区域淋巴结Lunx mRNA的表达,对入选患者进行为期3年的随访观察,并对6个可能影响NSCLC患者预后的因素进行Cox回归模型分析。结果:手术切除区域淋巴结Lunx表达率与年龄、营养指数、3年内的复发或转移率之间有相关性(P<0.01,P<0.01,P<0.05),而患者的性别、肺癌病理类型、分期与手术切除区域淋巴结Lunx表达率之间无相关性(P>0.05);NSCLC患者的生存时间与术后辅助治疗及与Lunx表达有统计学意义(P<0.05,P<0.01)。结论:NSCLC患者Lunx mRNA表达与其生存时间显著相关,提示Lunx mRNA可作为NSCLC淋巴微转移的分子标志物及监测患者3年预后的指标之一;同时术后辅助治疗是延长患者生存时间的一项重要措施;荧光实时RT-PCR相对定量方法可应用于NSCLC淋巴微转移的检测,该方法可能有助于早期肺癌转移的诊断。
Objective： To explore the mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer （NSCLC） and its correlation with prognosis. Methods SYBR green I real-time RT-PCR was used to detemine the lunx mRNA level in 153 regional lymph nodes obtained from 75 patients with NSCLC. All patients had been followed up for 3 years. Six factors that may influence the survival rate were analyzed by a Cox regression model. Results： Expressions of lunx in regional lymph nodes had a statistical correlation with age, nutritional index, relapse within 3 years and metastasis（ P 〈 0.01, P 〈 0.01, and P 〈 0.05） but had no correlation with gender, tumor types and pathological grades（ P 〉 0.05）. There were statistical significances between the survival of NSCLC patients and post-operative adjuvant therapy and expression of lunx （P 〈 0.05 and P 〈 0.01）. Conclusions： The expression of Lunx in regional lymph nodes closely correlates with the survival of NSCLC patients and it may be a target gene for detection of micrometastasis from NSCLC and a marker for detection in 3 years prognosis. Quantitative real-time RT-PCR is helpful in early diagnosis of micrometastasis of the lymph node in patients with NSCLC.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第9期882-885,共4页
Journal of Shandong University:Health Sciences
