摘要
目的研究X染色体STR单倍型DXS7424-DXS101在汉族群体中的法医学特征及其分布特点。方法本实验应用PCR和聚丙烯酰胺凝胶电泳及银染技术对DXS7424和DXS101位点进行基因分型,利用相关统计方法进行数据分析。结果汉族151个男性样本中共检出37种单倍型,频率分布在0.0066～0.1391,GD值为0.9453,DP值达到0.9389,最常见单倍型为16-23。结论DXS7424-DXS101作为单倍型分析在汉族群体中具有较高的个体识别率和非父排除率,在法医学特殊亲权鉴定中具有良好的应用前景。
Objective To validate the genetic characteristics and distribution of DXS7424-DXS101 on X chromosome in HaM population. Methods DXS7424 and DXS101 loci were genotyped by PCR, PAGE and silvers stain methods. Their genetic parameters were analyzed by Arlequin software. Results There were 37 haplotypes detected in 151 HaM unrelated males. The frequencies ranged from 0.0066 to 0.139 1, with a GD value of 0.9453 and a DP value of 0.9389. Haplotypes 16-23 were the most common haplotypes in HaM population. Conclusion Analysis of combined DXS7424 and DXS101 haplotypes appears to be a powerful means in population genetics and forensic practice for determination of identity and paternity.
出处
《法医学杂志》
CAS
CSCD
2007年第5期342-344,共3页
Journal of Forensic Medicine
基金
教育部科技基础平台项目:中华民族遗传资源数据整合共享平台(505015)
