摘要
以福建省34份余甘子遗传资源为材料,采用20个具特异扩增的多态性引物对福建省34份余甘子基因型进行扩增,20个引物,在34份供试材料中总共扩增出259个位点,且均是多态的,多态性程度达100%,平均每个引物扩增12.95个位点。同时,获得了19个引物对23份材料扩增的RAPD特征标记47个,17个引物在36对材料上共扩增出36个共享特征RAPD标记;采用ED(欧氏距离)法进行RAPD标记的聚类分析则可将福建34份余甘子遗传资源明显划分为惠安余甘和莆田余甘两大类。各供试材料之间的遗传距离范围为0.00～1.00,其中栽培品种与野生资源之间遗传距离在0.43～1.00之间;莆田余甘资源与惠安余甘资源之间遗传距离为0.27～0.99。
In this experiment 34 accessions of Phyllanthus emblica L. collected in Fujian Province were analyzed by RAPD technique. There were 259 sites totally detected by 20 random 10-mer oligo-mucleotide primers, all of which belonged to polymorphic sites with polymorphism being up to 100 %. Each primer produced 12.95 sites. In 23 accessions 47 RAPD characteristic markers were amplified with 19 primers, and 36 common characteristic RAPD markers within two cultivars or lines were amplified in 34 accessions with 17 primers. The 34 accessions of Fujian emblic germplasm were divided into 2 groups by clustering analysis with ED （Euclidean distance） method： one from Huian and the other from Putian. The genetic distances among the accessions were between 0.00 and 1.00, of which the genetic distances were 0.43-1.00 between cultivars and the wild, and 0.27-0.99 between Putian and Huian groups.
出处
《热带作物学报》
CSCD
2007年第2期74-79,共6页
Chinese Journal of Tropical Crops
基金
福建省科技重大专项(2004NZ02-2)资助项目的部分内容
关键词
余甘子
RAPD
主栽品种
野生资源
Phyllanthus emblica L. RAPD major cultivar clustering analysis genetic diversity
