摘要
目的:了解激肽释放酶基因表达与子痫前期发生、发展的关系。方法:用逆转录-聚合酶链反应(RT-PCR)及酶底物S2266分别检测子痫前期轻、重度患者共30例及30例正常孕妇(对照组)胎盘中激肽释放酶基因表达及尿液中激肽释放酶活性的差异。结果:与对照组相比,子痫前期患者胎盘中激肽释放酶mRNA的表达水平及尿液中激肽释放酶活性明显降低(P<0.01);而且子痫前期重度患者的上述指标较子痫前期轻度患者更低(P<0.05)。结论:激肽释放酶基因表达缺陷或抑制与子痫前期的发生、发展以及病情轻重相关。
Objective：To evaluate expression and activity of kallikrein in gravida with pre-eclampsia. Methods： Three groups were set as normal （30 cases ）, mild pre-eclampsia group （7 cases） and severe pre-eclampsia group（23 cases）. RT-PCR and S2266 was used to detect the expression of kallikrein mRNA in placenta and activity in urine respectively. Results：Expression level of kallikrein mRNA in placenta and the activity in urine from pre-ec- lampsia patients were significantly lower than those in control group （ P 〈0.01 ）. And in severe pre-eclampsia group, expression of kallikrein mRNA in placenta and activity in urine were also lower than those in mild group （ P 〈 0.05 ）. Conclusion： The repression and absence expres- sion of kallikrein are related to the severity of pre-eclampsia and may be involved in the devel- opment of this disease.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第9期667-669,共3页
Progress in Obstetrics and Gynecology
基金
广东省2002年医学科研基金资助项目(No:A2002623)
关键词
子痫前期
激肽释放酶
胎盘
尿液
Pre-eclampsia
Kallikrein gene
Placenta
Urine
