摘要
目的探讨不同的超声发射方式在微泡超声损伤肠系膜小血管中的效应。方法32只新西兰大白兔随机分为单纯微泡组、单纯超声辐照组、超声连续辐照微泡组和超声间歇辐照微泡组进行实验。静脉注射脂氟显微泡0.1ml/kg,用峰值声压2.6MPa的低占空比治疗超声照射,照射后肉眼观察照射区域肠系膜小动静脉血管损伤情况,取辐照区小血管做病理检查。结果单纯微泡组和单纯超声辐照组照射区小血管未发现任何生物学效应,而由微泡超声辐照的两组均出现了小血管破裂出血、血栓形成等生物学效应。肉眼观察超声间歇辐照微泡组形成的血肿大于超声连续辐照微泡组;病理学检查表明超声间歇辐照微泡组血管内皮细胞损伤,基底膜断裂以及血栓栓塞情况均高于超声连续辐照微泡组。且超声连续辐照微泡组形成的血管损伤情况不稳定。间歇辐照组损伤面积显著高于连续辐照组(P<0.01)。结论在适宜参数条件下,微泡超声空化可引起肠系膜小血管壁破裂出血、形成血栓及栓塞血管;微泡再灌注增强了血管的损伤。
Objective This study was to test the impact on small mesentery vascular induced by microbubble-mediated intermittent focused ultrasound. Methods Thirty-two New Zealand white rabbits were randomly divided into 4 groups for the factors including micmbubbles, ultrasound and ultrasound delivery modes（the intermittent mode and the continuous mode）. Focused ultrasound with a peak negative pressure of 2.6 MPa was delivered directly to mesentery vessels following intravenous injection of lipid microbubbles at 0.1 ml/kg. Hematoma happened around the vessels immediately when the insonation began. It was grossly measured in size then sent to pathological examination. Results In microbubble alone group and ultrasound exposure alone group,there was no biological effects on the small blood vessels. Severe red blood cell extrusion, endothelial malformation, discontinuity or rupture of artery wall and thrombosis happened in both the intermittent mode and the continuous mode when ultrasound and microbubbles were coordinated applied. However, the intermittent mode induced larger hematoma and more significant microscopic damage than the continuous mode （ P 〈 0.01 ）. Conclusion This preliminary study indicated ultrasound is able to make non-thermal mechanical damages to the mesentery small vessels when coordinated with microbubbles. The intermittent mode can induce more severe damage effects than that of the continuous mode.
出处
《临床超声医学杂志》
2007年第10期577-580,共4页
Journal of Clinical Ultrasound in Medicine
基金
国家自然科学基金(30471654)
关键词
微泡
空化效应
肠系膜小血管
Microbubbles
Cavitation
Mesentery small vessels
