摘要
目的探讨利用微分脉冲伏安技术测定全血中尿酸的电化学分析方法。方法玻碳电极在1mol/LNaOH溶液中活化,用循环伏安法研究尿酸在活化玻碳电极上的氧化还原特性,用微分脉冲伏安法直接测定尿酸的含量。结果在0.1mol/L的醋酸缓冲溶液中(pH5.0),尿酸在活化玻碳电极上于0.484V处产生一个灵敏的氧化峰。微分脉冲伏安法测定其氧化峰电流与尿酸的浓度在5.0×10-6～2.0×10-4mol/L范围内呈良好的线性关系,相关系数为0.9989,检出限为1.0×10-6mol/L。能在抗坏血酸存在下同时测定尿酸。结论方法操作简单方便,重现性较好,用于人血中尿酸的测定,结果令人满意。
Objective The electrochemical behavior of uric acid （UA） were investigated at AGCE by cyclic voltammetry （CV） and linear sweep voltammetry （LSV）. A novel method was proposed for the determination of UA in human whole blood by differential pulse voltammetry （DPV）. Methods The glassy carbon electrode was activated electrochemically in 1 mol· L^- 1 sodium hydroxide. The electrochemical behavior of UA were investigated at the activated glassy carbon electrode （AGOE） by cyclic voltammetry （CV）. The UA content was determined by differential pulse voltammetry （DPV）. Results A sensitive oxidation peak was observed at 0. 484 V （vs. SCE） in 0.1 mol/L HAc- NaAc buffer solution （pH 5.0） with 0.1 mol/L potassium chloride as supporting electrolyte. The peak current is well - proportional to the concentration of UA over the rang from 5.0 ×10^-6to 2.0 × 10^-4 mol/L by DPV, with the correlation coefficient of 0. 9989. Tt has been used to the determination of UA in the presence of ascorbic acid. Conclusion The activated glassy carbon electrode is easy to prepare with good reproducibility. The method was employed for measurement of UA in human whole blood, and satisfactory results were obtained.
出处
《济宁医学院学报》
2007年第4期284-286,共3页
Journal of Jining Medical University
关键词
活化玻碳电极
尿酸
血
循环伏安法
微分脉冲伏安法
Activated glassy carbon electrode
Uric acid
Blood
Cyclic voltammetry
Differential pulse voltammetry
