摘要
建立HPLC-ELSD法测定了30批银杏叶中4种银杏内酯的含量，并对所得数据进行分析研究。色谱条件为：phenonoononC18色谱柱（250mm×4．6mm，5μm），35％（体积分数）甲醇为流动相，1．000mL／min的流速，ELSD检测器漂移管温度为60℃，氮气压力为1．72×10^5Pa。得出红土栽培，未嫁接，树龄6～7a的银杏叶中银杏内酯含量较高；4种银杏内酯总量集中在0．3％～0．5％（质量分数）。该方法准确，重现性好，适用于银杏叶中4种银杏内酯的含量测定；银杏内酯的含量受生长环境影响差别较大，影响因素有待深入研究。
An HPLC-ELSD method was established to quantitatively determine four kinds of ginkgolides in 30 batches of ginkgo leaves, and the obtained data were analyzed. The analysis was performed on phenonoonon C18 column（250mm×4.6mm,5μm）with 35% methanol as mobile phase, at a flow rate of 1.000 mL/min. The drift tube temperature of the ELSD detector was 60 ℃ and the evaporative gas was nitrogen with pressure of 1.72 × 105 Pa. The results showed that the content of ginkgolides in ginkgo leaves, which grow in laterite for 6--7 years without graft, was higher. The total content of ginkgolides was always ranging 0.3 %--0.5 % . The method was accurate with good repeatability for the quantitative determination of ginkgolides in ginkgo leaves. The content of ginkgolides was largely affected by the growth conditions of ginkgo leaves and the influence factors needed to be deeply investigated.
出处
《化学工业与工程》
CAS
2007年第6期532-535,共4页
Chemical Industry and Engineering
