摘要
利用SignalP3.0、TMHMM2.0、TargetP1.01、LipoP1.0和PSORTb蛋白分析软件并结合L值计算,对植物病原细菌Ralstonia solanacearum GMI1000菌株基因组中的全部3440个ORFs进行了分析预测,确定其中186个ORFs所编码蛋白质的N-端有信号肽序列,且它们的氨基酸残基相对保守。其中134条具有分泌型信号肽,22条具有RR-motif型信号肽,30条具有信号肽酶Ⅱ型信号肽。对各类信号肽及其结构域的长度作了系统的分析。未发现Prepilin-like信号肽和细菌素和信息素信号肽。
The 3 440 open reading frames （ORFs） of Ralstonia solanacearum GMI1000 were used for predicting signal peptides by comprehensive analyses with SignalP 3.0, TMHMM 2.0, TargetP 1.01, LipoP 1.0 and PSORTb, and screening based on L value. Total 186 signal peptides with conserved amino acid residues are found and among them, 134 are secretary types, 22 are RR-motif types and 30 are lipoprotein signal peptides. The length distribution of signal peptide and its domains were analyzed systemically. No type Ⅳpilin-like signal peptides and bacteriocins and pheronones signal peptides are found in the database of deduced proteins encoded by the genome of R. solanacearum GMI1000.
出处
《遗传》
CAS
CSCD
北大核心
2007年第11期1409-1416,共8页
Hereditas（Beijing)
基金
重庆市自然科学基金项目(编号:8575)
重庆大学自然科学青年基金项目资助~~
