摘要
介绍利用油镜油观察花粉滚动和立体形状的制片方法,以及将普通显微镜（即明场显微镜）改制成暗场显微镜的方法和观察结果。将植物花粉均匀地撒在载玻片上,盖上盖玻片,用铅笔上的橡皮头等软物轻敲1~2下,然后在盖玻片外侧（或角端）滴上1小滴油镜油,油镜油沿着载玻片与盖玻片之间的缝隙向内扩散,制片完成并立即进行显微镜观察。利用油镜油对花粉进行制片,比较容易在40倍物镜下直观地观察和记录花粉的滚动过程和立体形状、层次结构、表面纹饰。将显微镜进行一些结构改建,可以一镜多用。随着光学显微镜的不断升级换代,光镜在观察花粉形状和结构信息等方面,将具有越来越多电镜无法取代的优势。
The study aimed to introduce a flaking method of using the immersion oil to observe the rolling and stereo shape of pollen,as well as the method of reorganizing common microscope（bright field microscope） into dark field microscope and the observation result.The microscope observation should be conducted at once after the flaking was finished by spraying botanic pollen on the microscope glass slide,covering the cover glass and knocking it for 1～2 times with soft things such as eraser on the pencil,then dropping a small drop of immersion oil on the outside or corner of cover slip,with the oil diffusing along the gap between microscope glass slide and cover glass into inside.The rolling course,stereo shape,hierarchy structure and surface ornamentation of pollen were easier to observe and record visually under 40-fold microscope by flaking pollen with immersion oil.A multi-use microscope could be made by doing some structural remodeling on the microscope.With continuous upgrade of optical microscope,it would have more and more advantages which couldn＇t be replaced by electrical microscope on aspects such as observing pollen shape and structure information.
出处
《安徽农业科学》
CAS
北大核心
2007年第30期9464-9464,9469,共2页
Journal of Anhui Agricultural Sciences
关键词
光学显微镜
花粉
滚动
立体形状
制片
Optical microscope
Pollen
Rolling
Stereo shape
Slide making
