摘要
研究选用了几种商业蛋白酶对乳清蛋白浓缩物(WPC)进行酶解,通过比较各酶解产物中α-乳白蛋白(α-La)和β-乳球蛋白(β-Lg)存余率的高低,筛选出Protease A具有优先降解β-Lg的能力;通过单因素实验设计和正交实验设计优化了Protease A优先降解β-Lg的工艺。最佳工艺为温度40℃,pH值为7.3,E/S为800U/g蛋白,酶解时间为3h,此时水解物的水解率(DH)为8.40%,α-La和β-Lg的存余率分别为5.3%和1.4%,水解产物的溶解性得到了明显地改善(P<0.05)。
Several proteases were screened to hydrolyze WPC. By comparing the percentage of α-lactalbumin （α-La） and β-lactoglobulin （β-Lg） unhydrolyzed in the solutions, the result showed that Protease A hydrolyzed β-Lg preferentially in WPC solution. Furthermore, the optimum conditions of hydrolysis （T=40 ℃, pH=7.3, E/S=800 U/g and incubating time=3 h） were determined by single factor experiment design and orthogonal experiment design. The final DH was 8.40%, the percentage of α-La and β-Lg unhydrolyzed were 5.3% and 1.4% respectively, and the solubility was improved remarkably （P〈0.05）.
出处
《中国乳品工业》
CAS
北大核心
2007年第10期4-7,共4页
China Dairy Industry
