摘要
目的对福建畲族群体15个短串联重复序列等位基因遗传多态性进行分析,探讨其种族特异性及其在法医学个体识别和亲权鉴定的中的应用。方法荧光标记15个短串联重复序列(STR)位点和一个性别位点并进行PCR复合扩增,用ABI遗传分析仪以毛细管电泳技术对100例福建畲族无血缘关系健康个体PCR产物进行电泳分离基因,并以分析软件进行基因分型。结果共检出126个等位基因,基因频率范围在0.0050～0.5550。15个STR基因座的基因型分布均符合Hardy-Weinberg平衡。15个STR位点的HET在0.6300～0.8900之间,DP在0.7680～0.9590之间,EPP在0.3280～0.7750之间,PIC在0.5200～0.8600之间。累积DP和EPP分别达到0.99999999和0.99999288。结论15个STR位点均有较高的遗传多态性,并有丰富的信息含量,适合作为福建畲族地区的遗传标记,可用于个体识别和亲权鉴定。
Objective To analyze the genetic polymorphism of 15 short tandem repeat （STR） loci and forensic applications in Fujian She population. Methods Fluorescence-labeling polymerase chain reaction and capillary electrophoresis were used to analyze the genetic polymorphism of 100 randomly selected individuals from Fujian She population at 15 STR loci. Results All the 15 loci met Hardy-Weinberg equilibrium. There are 126 STR alleles in Fujian She population, with allelic frequencies ranging from 0. 0050 to 0. 5550, heterozygosity （HET） from 0. 6300 to 0. 8900, discrimination power （DP） from 0. 7680 to 0. 9590, probabilities of paternity exclusion （EPP） from 0. 3280 to 0. 7750 and polymorphic information content （PIC） from 0. 5200 to 0. 8600. The accumulative DP and EPP were 0. 99999999 and 0. 99999288 respectively. Conclusion The highly genetic polymorphism is observed in Fujian She population. The fifteen STR loci can meet the needs of the parentage testing and personal identification in forensic medicine.
出处
《福建医药杂志》
CAS
2007年第5期9-12,共4页
Fujian Medical Journal
基金
福建省医学创新课题(2003-CX-14)
