期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

血管活性肠肽对大鼠Leydig细胞的免疫调节作用 被引量：6

Immunoregulation of vasoactive intestinal peptide on rat Leydig cells
原文传递
导出
摘要 目的:研究血管活性肠肽(VIP)对大鼠睾丸间质细胞(Leydig细胞)免疫功能的调节。方法:以VIP作用于溶脲脲原体(UU)感染的SD大鼠及大鼠Leydig细胞为研究对象,观察VIP对大鼠Leydig细胞分泌的细胞因子(IL-1、IL-6及TGF-β)和FasL表达的调节,从体外及动物整体水平来探讨VIP对大鼠Leydig细胞免疫功能的调节。结果:在睾丸局部感染时,VIP能调节Leydig细胞IL-1、IL-6、TGF-β及FasL的分泌和表达格局;同时VIP对大鼠睾丸局部免疫豁免的调节和维持也起到一定的作用。结论:VIP能调节大鼠Leydig细胞的免疫功能,并参与调节大鼠睾丸局部的免疫豁免。 AIM： To study the regulation of immune function of rat Leydig cells by vasoactive intestinal peptide （VIP）. METHODS： Rat Leydig cells were seperated and infected by UU, with or without the incubation with VlP. The expression of FasL on Leydig cells in different group was analysed by flow cytometry （FCM）. The mRNA expression of IL-1, IL-6, TGF-13 and FasL of Leydig cells in different group was identified by RT-PCR. SD rats were infected by UU with or without the injection of VIP, and the testis tissnes of each group were har vested and observed under transmission electron microscope. RESULTS： When testis was infected by UU, VIP up-regulated the mRNA expression of IL-1, IL-6, and TGF-13, and down-regulated the expression of FasL in vitro. In addition, there was significant difference in testis rnorphous of rats from different group. CONCLUSION： VIP could regulate the expression pattern of IL-1, IL-6, TGF-13 and FasL by Leydig cells, which may contribute to maintain immune privilege of the testis. VIP could regulate rat Leydig cell immune function.
作者 王伟 席晔斌 陈广洁 郝静 沈天伟 王保国 李伟毅
机构地区 上海交通大学医学院上海市免疫学研究所
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2007年第8期696-700,共5页 Chinese Journal of Cellular and Molecular Immunology
基金 上海市教委科研基金资助(04BB21) 上海市重点学科建设项目资助(T0206)
关键词 LEYDIG细胞 免疫调节 IL-1 IL- 6 TGF-β FASL Leydig cell immunoregulation IL-1 IL-6 TGF-13 FasL
分类号 R329.12 [医药卫生—人体解剖和组织胚胎学] R392.23 [医药卫生—免疫学]
  • 相关文献

参考文献12

  • 1Rayne AH.The Leydig cell[M].Vienna:Cache River press,1996:231-506.
  • 2Cudicini C,Lejeune H,Gomez E,et al.Human leydig cells and sertoli cells are produc ers of interleukin-1 and-6[J].J Clin Endocrinol Meteb,1997,82(5):1416-1433.
  • 3Le Roy C,Lejeune H,Chujel F,et al.Autocrine regμlation of leydig cell differenfiated functions by insulin-like growth factor I and transforming growth factor beta[J].J Steroicl Biochem Mol Biol,1999,69(1-6):379-384.
  • 4Sandro F,Peira D,Nadia R,et al.Fas and Fas Ligand expression in fetal and adult human testis with normal or deranged spermatogenesis[J].J Clin Endocrinol Metab,2000,85(8):2692-2700.
  • 5张颖德,李伟毅,席晔斌,陈广洁,王保国,包士三.脲原体感染大鼠Leydig细胞时IL-6、Fas和FasL表达的变化[J].免疫学杂志,2004,20(2):95-99. 被引量：6
  • 6Voice J,Grinninger C,Kong Y,et al.Roles of vasoactive Intestinal Peptide (VIP) in the expression of different immune phenotypes by wild-type mice and T cell-targeted type II VIP receptor transgenic mice[J].J Immunol,2003,170(1):308-314.
  • 7席晔斌,王保国,陈广洁,包士三,李伟毅.大鼠Leydig细胞分泌的IL-1和TGF-β_1在感染时的免疫调节作用[J].生殖与避孕,2002,22(5):309-312. 被引量：4
  • 8徐晨,孙广芳,徐胜,王一飞.溶脲脲原体感染动物模型的建立─对睾丸的形态学影响[J].生殖与避孕,1995,15(3):205-209. 被引量：35
  • 9Mullol J,Baraniuk JN,Pitale M,et al.Vasoactive intestinal peptide (VIP) induces IL-6 and IL-8,but not G-CSF and GM-CSF release from a human bronchial epithelial cell line[J].Neuropeptides,1997,31(2):119-124.
  • 10Delgado M,Ganea D.Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibit expression of Fas ligand in activated T lymphocytes by regulating c-Myc,NF-kappa B,NF-AT,and early growth factors 2/3[J].J Immunol,2001,166(2):1028-1040.

二级参考文献17

  • 1李伟毅,程丽萍,朱云凤,徐晨,徐胜,王一飞.大鼠泌尿生殖系溶脲脲原体感染的病理变化[J].上海第二医科大学学报,1996,16(5):350-352. 被引量：11
  • 2Cudicini C, Lejeune H, Gomez E, et al. Human leydig cells and Sertoli cells are producers of interleukins-1 and -6 [J]. J Clin Endocrinol Metab, 1997, 82 (5): 1 426 - 1 433.
  • 3Le Roy C, Lejeune H, Chuzel F, et al. Autocrine regulation of leydig cell differentiated functions by insulin-like growth factor I and transforming growth factor beta [ J ]. J Steroid Biochem Mol Biol, 1999, 69 (1/6): 379 - 384.
  • 4Voice JK, Grinninger C, Kong Y, et al. Roles of vasoactive intestinal peptide (VIP) in the expression of different immune phenotypes by wild-type mice and T cell-targeted type Ⅱ VIP receptor transgenic mice [J]. J Immunol, 2003, 170 (1):308 - 314.
  • 5Han XD, Wang Y, Chen JX. A comparative study on interrelations among microelements, infection of Ureaplasma urealyticum, and male infertility [ J]. Arch Androl, 2003,49 (4): 265 - 269.
  • 6Audring H, Klug H, Bollmann R, et al. Ureaplasma urealyticum and male infertility: an animal model: Ⅱ.Morphologic changes of testicular tissue at light microscopic level and electron microscopic findings [ J ]. Andrologia,1989, 21(1): 66-75.
  • 7Millette CF, Laufer MR, Owens N, et al. Serological and biochemical identification of a plasma membrane antigen specific to leydig cells [J]. Biol Reprod, 1984, 31 (5):1 037-1 048.
  • 8王学萍 杨明久.检测白介素1（IL—1）三种方法的初步探讨[J].中国免疫学杂志,1987,3(1):26-28.
  • 9Juarranz MG, Santiago B, Torroba M, et al. Vasoactive intestinal peptide modulates proinflammatory mediator synthesis in osteoarthritic and rheumatoid synovial cells [J]. Rheumatology (Oxford), 2004,43(4) :416 - 422.
  • 10Martinez C, Delgado M, Pozo D, et al. Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide modulate endotoxin-induced IL-6 production by murine peritoneal macrophages [J]. J Leukoc Biol, 1998, 63 (5): 591 -601.

共引文献44

同被引文献103

引证文献6

二级引证文献14

细胞与分子免疫学杂志
2007年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部