摘要
目的:检测生长激素(GH)在体内对胆管细胞癌(QBC939)的作用,探讨胰岛素样结合蛋白3(简称IGFBP3)可能作用机制。方法:将QBC939培养后接种裸鼠,成瘤后随机分为注射不同剂量、时间的GH组(GH组,又分为4mg2周、2mg2周、2mg3周3个亚组)和生理盐水组(NS组),分别测荷瘤鼠体重、瘤重和瘤重/尸重比;流式细胞仪检测肿瘤细胞周期;Westernblot测定肝脏IGFBP3。结果:实验组与对照组荷瘤鼠体重、瘤重和瘤重/尸重比,种植瘤细胞周期、增殖指数(PI)等指标,各组间均无统计学差异(P>0.05)。与对照组相比,实验组荷瘤鼠肝脏IGFBP3表达显著增强(P<0.01),且随剂量加大上调效应更为明显。结论:GH在体内不刺激QBC939生长,其机制可能与IGFBP3的作用有关。
Objective：To explore the effect of recombinant growth hormone on cholangiocarcinoma in vivo,and study the possible mechanism of IGFBP3. Methods：Athymic nude mice were injected with QBC939 cells. When tumor became palpable obviously,animals were randomized to receive GH（GH groups：4 mg 2w,4 mg/kg.s.c. once daily for 2 weeks;2 mg 2w,2 mg/kg.s.c. for 2 weeks;2 mg 3w,2 mg/kg.s.c. for 3 weeks） versus saline control（NS groups）. Animals and tumour were weighed,tumor and carcass weight rate were determined. Cells of tumor tissues were evaluated by flow cytometry（FCM）. Western blot was used to detect IGFBP3 in mice liver. Results：There was no statistical significance of the difference on the host features（body weight,tumor weight,tumor and carcass weight rate,P 〉 0.05） or the cell cycle kinetics（G1,G2M,S,PI,P 〉 0.05） between GH groups and NS groups in vivo. Compare with NS groups,the expression of IGFBP3 in GH groups in mice liver was significantly increased according to the dose of GH[（2 mg/（kg·d）：（0.63 ± 0.05） vs （0.28 ± 0.05）,and 4 mg/（kg·d）：（0.71 ± 0.07） vs（0.28 ± 0.05）,respectively,P 〉 0.01]. Conclusion：GH couldn＇t accelerate tumor growth in vivo,and the possible mechanism was acted by the effect of IGFBP3.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期936-938,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅135基金资助(135-08)
