摘要
目的:观察脑维康(NWK)对D-半乳糖致脑老化大鼠模型学习记忆障碍的影响。方法:大鼠背颈部皮下连续注射D-半乳糖生理盐水(80 mg/kg)8周建立脑老化大鼠模型,灌胃给予NWK 8周。以Morris水迷宫试验检测模型大鼠的空间学习记忆能力,以分光光度法检测乙酰胆碱酯酶(acetylcholinesterase,AChE)、黄嘌呤氧化酶法测定过氧化物歧化酶(superoxide dismutase,SOD)、硫代巴比妥酸法测定丙二醛(maleic dialdehyde,MDA)的变化,以HE染色进行脑组织形态学观察。结果:D半-乳糖致脑老化大鼠在水迷宫中的游泳持续时间、路径长度明显延长。给药8周后,NWK 23 mg/kg、46 mg/kg组大鼠游泳持续时间、路径长度较模型组明显缩短,搜索策略均有改进。与空白组比较,模型组大鼠全脑AChE活力、MDA含量均明显升高,SOD活力明显降低。给药8周后,与模型组大鼠相比,NWK各给药组大鼠全脑AChE活力明显降低,NWK 46 mg/kg组大鼠全脑MDA含量明显降低,脑组织形态结构有所改善。结论:NWK可改善D-半乳糖致脑老化大鼠模型学习记忆能力。
Objective： To study the protective effect of a combination of extracts of Chinese medicines （ Naoweikang, NWK） on learning and memory dysfunction in rat aging models induced by D-galactose and the potential mechanisms. Methods ： A 2-month NWK （23 mg/kg and 46 mg/ kg, respectively） administration to rats was performed daily after a 2-month subcutaneous injection of D-galactose （80 mg/kg, 10 mL/kg）. Assisted with the exploring strategy, the escape latency and swimming distance of rats within 3 min were adopted as the main parameters to detect the effect of NWK on learning and memory dysfunction. AChE activities were determined with spectrophotometry, SOD activities with xanthine oxidase method and MDA contents with thibabituric acid method, respectively. Histological changes in mouse brain tissue were observed after HE stain. Results： Compared with shams, the escape latency and swimming distance of models were prolonged significantly; and SOD activities in whole brains significantly decreased while MDA contents increased significantly. Compared with models, the escape latencies or swimming distances of rats in NWK-administrated groups （23mg/kg and 46mg/kg, respectively） shortened significantly and the exploring strategies were improved; AChE activities in whole brains of rats in each NWK-administrated group and MDA contents of rats in 46mg/kg-administrated group decreased significantly. Conclusion： NWK significantly improved learning and memory dysfunction in rat aging models induced by D-galactose. The mechanisms might be due to three points below： inhibiting central AChE activities therefore to reduce the degradation of ACh and increase the level of central ACh; increasing the ability of antioxidation and clearing free radicals therefore to protect the neurons; protecting the morphological structures of brain tissues.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第5期155-158,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家"863"高技术研究发展计划资助项目(2002AA2Z346B)。
关键词
脑维康
D半-乳糖
学习记忆
Naoweikang （ NWK, 脑维康 ）
D-galactose
learning and memory
aging rat
