摘要
枯草芽孢杆菌B9601-Y2是一个具有防治植物病害和促进植物生长的专利菌株。本文采用Sau3A I部分酶切的DNA、粘粒载体pLARF-5和PackageneλDNA包装系统,构建了该菌株基因组文库。文库总量为11 000个克隆,插入片段平均长度为14.77 kb,按99.99%的概率计算,文库覆盖了基因组4.2次。这为该菌株防治病害和促进植物生长机理研究奠定了物质基础。
Bacillus subtilis B9601 - Y2 is a patented bioagent controlling fungal diseases and promoting growth of plants. Its genomic DNA library was constructed by partial digestion with Sau3A Ⅰ and cosmid vector pLAFR-5 following the Packagene Lambda DNA Packaging System. The library contains 11 000 clones with an average of 14. 77 kb insert, covering 4.2 genomic equivalences under 99.99% probability, which provides with basis the research on mechanisms of controlling diseases and promoting growth for the bacterium.
出处
《云南农业大学学报》
CAS
CSCD
2007年第6期799-801,807,共4页
Journal of Yunnan Agricultural University
基金
国家自然科学基金(30660007)
