摘要
以五氧化二磷、甲烷磺酸为反应试剂,均相制备磷酸化壳聚糖;红外光谱、核磁共振显示其磷酸根的取代位为6位羟基,耦合等离子发射光谱测试磷酸化壳聚糖的取代度为0.22,比浊法测定等电点为pH 5.0.磷酸化壳聚糖和海藻酸通过静电作用制成复合膜后,石英微晶天平(QCM)评价膜对血液中纤维蛋白原、白蛋白及血小板的吸附作用,与单纯壳聚糖膜相比,复合膜对纤维蛋白原和血小板的吸附量减少,对白蛋白的吸附量增多.磷酸化壳聚糖与海藻酸复合膜具有一定抗凝血性的特征,为以后进一步研究工作奠定基础.
6-hydroxyl phosphonic chitosan （p-CS） was synthesized in a homogeneous phase system in which phosphorus pentoxide and methanesulphonic acid were added. The p-CS obtained was character- ized by FTIR, C13-NMR and XRD. The results showed that the location of phosphate radical was 6-hydroxyl and crystalline was decreased after phosphorylated. The degree of substitution was 0.22 by ICP testing and isoelectric point of p-CS was determined as pHS. 0 by turbidimetry. P-CS and alginate were made into compound films through electrostatic interaction. The potential of the films to absorb fibrinogen, albumin and plasma were evaluated by quartz crystal microbalance （QCM）. Compared with the chitosan films, those compound membranes could absorb more fibrinogen, plasma and fewer albumin. The compound membranes indicated good anticoagulant property and it was a base for studying the biological function in the next research.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2007年第5期498-502,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省十五重大专项(A302020104)
关键词
磷酸化壳聚糖
海藻酸
蛋白吸附
微晶天平
chitosan
phosphonic chitosan
alginate
quartz crystal microblance
