摘要
目的：探讨ＩＬ６在系膜增殖性肾炎（ＭｓＰＧＮ）发病中的作用。方法：采用逆转录聚合酶链式反应方法检测６例ＭｓＰＧＮ、５例非ＭｓＰＧＮ患儿肾活检组织及４例正常肾组织中ＩＬ６ｍＲＮＡ的相对表达水平。结果：ＭｓＰＧＮ、非ＭｓＰＧＮ患儿肾组织中ＩＬ６ｍＲＮＡ表达水平（ｘ±ｓ）分别为：０４３３±０１１１和０２７１±００２３。ＭｓＰＧＮ组ＩＬ６表达水平明显高于非ＭｓＰＧＮ组（Ｐ＜００２）。４例正常肾组织中３例无ＩＬ６ｍＲＮＡ表达，余１例表达水平为０１９２。中度ＭｓＰＧＮ和轻度ＭｓＰＧＮ表达水平分别为：０４９１±００８４和０３１７±００１８，二者比值为１５∶１，ＭｓＰＧＮ伴肾小球局灶节段硬化和不伴硬化组其表达水平分别为０５１６±００８３和０３５０±００５８，二者比值为１４７∶１。另采用生物活性法测定ＭｓＰＧＮ患儿尿中ＩＬ６活性水平，发现尿中ＩＬ６活性与肾组织中ＩＬ６ｍＲＮＡ的表达水平明显正相关（ｒ＝０９７７，Ｐ＜０００１）。结论：ＩＬ６可能与ＭｓＰＧＮ发病机理有关，ＩＬ６可能参与ＭｓＰＧＮ系膜增殖和硬化。
OBJECTIVE Studies in vitro has revealed the effect of IL 6 in the pathogenesis of mesangial proliferative glomerulonephritis(MsPGN). In this study, we detected IL 6 mRNA expression in renal tissue of children with MsPGN and to analysis of the relationship between the renal mRNA expression and the urinary activity or the related pathological changes. METHODOLOGY RT PCR assay was used to detect IL 6 mRNA expression in renal biopsy tissues in 6 patients with MsPGN, 5 patients with non MsPGN. Tissue from 4 non diseased kidney were used as control. RESULTS Relative IL 6 mRNA expression levels ( ±s ) detected by biological activity method in patients with MsPGN and non MsPGN were 0.433±0.111 and 0.271±0.023, respectively. The IL 6 mRNA expression level in patients with MsPGN is much higher than that in patients with non MsPGN ( P <0..02). Tissues from three normal kidney specimen showed no IL 6 mRNA expression. MsPGN patient with moderate proliferation or with sclerosis tend to have higher level of IL 6 mRNA expression than those patients without. There was positive correlation between urinary IL6 activity and renal IL 6 mRNA expression in MsPGN( r =0.977, P <0.001) CONCLUSION IL 6 plays a role in the pathogenesis of MsPGN and may be involved in mesangial proliferation and sclerosis in patients with MsPGN. Urinary IL 6 activity in patients with MsPGN might be a reflection of the renal IL 6 mRNA expression.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1997年第3期232-236,共5页
Chinese Journal of Nephrology,Dialysis & Transplantation
基金
国家自然科学基金
