摘要
目的:为建立人多发性骨髓瘤(Multiple Myeloma,MM)荷瘤SCID小鼠模型,研究人多发性骨髓瘤在SCID小鼠体内生长的特性。方法:体外培养IL-6依赖的人MM细胞株XG7,接种于SCID小鼠皮下;观察肿瘤生长状态,通过流式细胞技术监测肿瘤细胞表型。结果:成瘤后的肿瘤细胞CD28、CD138等分子的表达与接种前没有明显变化。结论:通过研究人MM细胞的体内生长特性可为MM的治疗奠定良好的基础。
Objective： For the establishment of human multiple myeloma （Multiple Myeloma. MM） tumor model in SCID mice, vivo growth characteristics of multiple myeloma in SCID mice were studied. Methods： MM cell lines XG7 depended on IL-6 were cultivated in vitro, then cells were collected to be inoculated below the skin of SCID mice; tumorigenic state and phenotype of tumor cells were observed by flow cytometry. Results： The results showed that the expression of molecular makers of the tumor cell such as CD28 and CD138 had no significant changes before inoculation. Conclusion： So the study of vivo growth characteristics of human MM cells plays an important role in the cure of MM.
出处
《实用医技杂志》
2007年第22期2964-2965,共2页
Journal of Practical Medical Techniques
