摘要
目的:研究Toll样受体9(TLR-9)在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)上的表达水平及SLE患者血清白介素-10水平,探讨发病机制。方法:从23例活动期、19例缓解期SLE患者和20例正常对照组中分离PBMCs,利用反转录-聚合酶链反应(RT-PCR)法检测PBMCs中TLR9 mRNA的表达水平,利用酶联免疫吸附试验法检测其血清白介素-10水平。结果:活动期SLE患者PBMCs的TLR-9mRNA表达高于缓解组(P<0.01)及正常对照(P<0.01),缓解期和正常对照组相比,差异无统计学意义(P>0.05)。SLE活动期患者血清IL-10水平显著高于缓解期患者(P<0.01),并均高于正常对照组(P<0.01)。结论:活动期SLE患者PBMC的TLR9 mRNA的表达水平增高;并且活动期及缓解期SLE患者血清IL-10水平升高可能与TLR9 mRNA表达的上调相关。
Objeetive： To study the expression of toll like receptor 9 （TLR-9） mRNA on peripheral blood mononuclear cells （PBMCs） and interleukin 10 （ IL- 10） levels in serum of patients with systemic lupus erythenmtosus （SLE）, and to discuss the potential mechanism, Methods： The expression ofTLR-9 mRNA on PBMCs of 42 SLE patients （23 in active stage and 19 in inactive stage） and 20 health controls were determined by reverse transcription polymerase chain reaction （RT-PCR）, the serum levels oflL-10 were determined by ELISA in each group. Results： Compared to inactive patients and healthy controls, the expression of TLR-9 on PBMCs of patients in active stage significantly increased （P〈0.01 ）. The serum levels of IL-10 in active SLE patients was significantly higher than the inactive patients （P〈0.01）, and that of the two groups patients were both higher than the health controls（P〈0.01 ）. Conclusions： The expression of TLR-9 mRNA on PBMCs of active SLE patients increased significantly. The increase of IL-10 levels of active and inactive SLE patients may be correlative with the high expression of TLR-9 mRNA.
出处
《现代生物医学进展》
CAS
2007年第11期1698-1700,共3页
Progress in Modern Biomedicine
