摘要
针对磨盘柿杂种胚挽救培养后的幼苗侧根少、直接成苗率低这一问题,采用对比法设计研究了在后续培养中几种措施的生根壮苗效应。结果表明:在原萌发培养基中添加1%墨汁可使胚挽救培养的直接成苗率提高到87%以上;对不能直接驯化移栽的胚挽救苗,短截主根和无糖培养措施能促进其侧根发育,并提高了幼苗的自养能力;3种措施的综合运用最终可使96%以上的杂种胚得以挽救成苗,并成功移栽于大田。
Rooting and hardening-off culture were specially performed to solve some problems emerged in previous embryo rescue of Chinese persimmon cv. Mopanshi. The contrast designs showed that adding 1% Chinese ink in the basic medium for germination could raise the percentage of plantlet formation by 87 %. In hardening-off culture stage, cutting the main root and sucrose-free culture could improve the development of lateral roots and the nutrient acclimation of hybrid plantlets. By combining use of above three measures, more than 96 % embryos were successfully rescued and transplanted to field.
出处
《华北农学报》
CSCD
北大核心
2007年第5期117-119,共3页
Acta Agriculturae Boreali-Sinica
基金
北京市自然科学基金项目(6052013)
河南科技学院博士基金项目(06010)
关键词
磨盘柿
胚挽救
生根壮苗培养
Mopanshi persimmon
Embryo rescue
Rooting and hardening-off culture
