摘要
目的探讨产生两种小鼠恐惧记忆差异的原因。方法选用昆明种（KM）和C57BL/6小鼠,测定血浆皮质酮水平以及检测其差异,并与行为学结果进行相关性比较。结果KM小鼠对照组和训练组血浆皮质酮水平分别为（101.083±70.266）μg/L、（215.453±60.276）μg/L,两者比较差异有显著性（P〈0.01）;训练组血浆皮质酮水平与训练结果木僵反应时间（36.00±29.292）s之间无相关性（r=-0.273,P〉0.05）。C57BL/6小鼠对照组和训练组血浆皮质酮水平分别为（108.009±20.452）μg/L、（176.933±38.202）μg/L,两者比较差异有显著性（P〈0.01）;训练组血浆皮质酮水平与训练结果木僵反应时间（257.50±36.450）s之间无相关性（r=-0.462,P〉0.05）。两种小鼠对照组、训练组比较,差异无显著性（P〉0.05）。结论KM小鼠与C57BL/6小鼠恐惧记忆差异可能与血浆皮质酮水平无关。
Objective To clarify the roles of hypothalamus-hypohysis-adrenocortical axis played in the interstrain difference in the retention of fear memory in two strains of mice, Kunming mice （ KM ） and C57BL/6. Methods The conditioning was to associate a footplate shock with a tone signal. The duration of tone-induced stupor response 24-hr after the training represents the retention of fear memory. Serum corticosterone （CORT） level was assayed using HPLC method. Results In KM, serum CORT concentration was（215. 453 ± 60.276）μg/L in trained group, significantly higher than that in the control group （ 101. 083±70. 266）μg/L, P〈0.01 ）. In C57BL/6, tserum CORT concentration in trained group and the control group were （ 176. 933 ± 38. 202 ） μg/L and （108. 009 ±20. 452）μg/L respectively（ P〈 0.01 ）. In trained KM, stupor response duration was （36.00 ± 29. 292） s, and in trained C57BL/6, the duration was （257.50 ± 36. 450） s, which were not correlated to the corresponding serum CORT levels（ r = - 0. 462, P 〉 0.05 and r = - 0. 273, P 〉 0.05 respectively）. Conclusion Fear conditioning induced comparable stress responses in KM and C57BL/6 mice;and serum CORT level might not account for the interstrain difference in retention of fear memory in these two strains.
出处
《中国行为医学科学》
CSCD
2007年第11期966-968,共3页
Chinese Journal of Behavioral Medical Science
关键词
恐惧记忆
应激
皮质酮
动物模型
Fear memory
Stress
Corticosterone
Animal model
