期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

丙泊酚对缺血再灌注诱导心肌细胞凋亡的影响 被引量：2

Effect of propofol on myocardial apoptosis induced by ischemia-reperfusion
下载PDF
导出
摘要 目的探讨丙泊酚对大鼠心肌缺血/再灌注(MI/R)后心肌细胞凋亡的影响及其作用机制。方法选用250-300g雄性SD大鼠,制备大鼠MI/R模型[3],给以45min缺血和2h、4h再灌注。根据实验目的,将45只SD大鼠随机均分为3组(n=15)。假手术组(sham组):在冠状动脉前降支(LAD)下穿线,但不结扎,其余操作与其他各组相同;单纯MI/R组(MI/R组):大鼠接受MI/R处理;丙泊酚组(P组):大鼠接受MI/R处理,在再灌注期间给以0.42mg/(kg.h)丙泊酚。再灌注结束后采用DNA原位末端缺口标记技术(TUNEL)测定心肌细胞凋亡比例,免疫组化法测定心肌细胞内抗凋亡分子Akt、Bcl-2和促凋亡分子p38MAPK的表达。结果与假手术组比较,丙泊酚组MI/R诱导的心肌组织的凋亡明显降低(P<0.05)。免疫组化的结果显示,丙泊酚组肌内Akt和Bcl-2的形成增加,p38MAPK的水平降低(P<0.05)。结论本实验结果提示丙泊酚对MI/R后的心肌组织有显著的保护作用,它的这种作用与其对Akt、Bcl-2和p38MAPK的影响密切相关。 Objective To explore the effect of propofol on myocradial apoptosis induced by myocardial ischemia-reperfusion（MI/R） and its potential mechanism. Methods Forty-five Wistar rats weighing 250 - 300 g were randomly divided into three groups： sham operation group （0.9% NaCl） ;MI/R group; propofol group [intravenous infusion 0.42 mg/（kg·h） during reperfusion]. At the end of reperfusion, the ratio of myocardial apoptosis was analyzed with TUNEL assay, and the expression of pAkt, p38 MAPK and Bcl-2 was detected with immunohistochemical staining. Results Compared with sham operation group, myocardial apoptosis decreased significantly in propofol group. The expression of pro-apoptotic p38 MAPK decreased in propfol group, but the expression of antiapoptotic proteins Akt and Bcl-2 significantly increased in cadiomyocytes. Conclusion These results indicate that propofol is more likely to protect cardiomyocytes from the injury of MI/R-induced apoptosis by increasing production of anti-apoptotic proteins.
作者 张彦清 岳毅 陈斌
机构地区 山西医科大学第一临床医学院麻醉科 山西武警总院神经外科 大同市第二人民医院骨科
出处 《山西医科大学学报》 CAS 2007年第11期965-967,1055,共4页 Journal of Shanxi Medical University
关键词 再灌注损伤 丙泊酚 凋亡 reperfusion injury propofol apoptosis
分类号 R364.1 [医药卫生—病理学]
  • 相关文献

参考文献11

  • 1Chang H,Tsai SY, Chang Y, et al. Therapeutic concentrations of propofol protects mouse macrophages from nitric oxide-induced cell death and apoptosis [ J ]. Can J Anaesth, 2002,49 : 477 - 480.
  • 2Acquaviva R, Campisi A, Murabito P, et al. Propofol attenuates peroxynitrite-mediated DNA damage and apoptosis in cultured astrocytes: an alternative protective mechanism[J]. Anesthesiology,2004,101 : 1363 - 1371.
  • 3Feng Gao, Erhe Gao, Xin Liang Ma. Nitric oxide mediates the antiapoptotic effect of insulin in myocardial ischemia-reperfusion[ J ]. Circulation, 2002,105 : 1497 - 1502.
  • 4Liang F, Gao EH, Tao L, et al. Critical timing of L-arginine treatment in post-ischemic myocardial apoptosis role of NOS isoforms[ J ]. Cardiovasc Res,2004,62 : 568 - 577.
  • 5Nam SY,Jung GA,Hur GGC, et al. Upregulation of FLIP(S)by Akt, a possible inhibition mechanism of TRAIL-induced apoptosis in human gastric cancer[J]. Cancer Sci, 2003,94: 1066- 1073.
  • 6Kim AH, Khursigara G, Sun X, et al. Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1 [J ]. Mol Cell Biol, 2001, 21:893-901.
  • 7苗丽君,王静.Akt与肿瘤的研究进展[J].国外医学（生理病理科学与临床分册）,2004,24(5):406-409. 被引量：38
  • 8Barata JT,Cardoso AA,Boussiotis VA. Interleukin-7 in T-cell acute lymphoblastic leukemia:an extrinsic factor supporting leukemogenesis[J]? Lank Lymphoma,2005,46(4):483-495.
  • 9Riggins RB, Bouton AH, Liu MC, et al. Antiestrogens, aromata- inhibitors, and apoptosis in breast cancer[J ]. Vit Horm, 2005, 71 : 201 - 237.
  • 10Giovannetti E,Mey V,Nannizzi S,et al. Pharmacogenetics of anticancer drug sensitivity in pancreatic cancer [ J ]. Mol Cancer Ther,2006,5(6) : 1387-1395.

二级参考文献20

  • 1Potter C J, Pedraza LG, Xu T. Akt regulates growth by directly phosphorylating Tsc2 [ J ]. Nat Cell Biol, 2002,4 (9) :658-665.
  • 2Vander Heiden MG, Plas DR, Rathmell JC, et al . Growth factors can influence cell growth and survival through effects on glucose metabolism[J]. Mol Cell Biol, 2001 ,21 (17) :5899-5912.
  • 3Edinger AL, Thompson CB. Akt maintains cell size and survival by increasing mTOR-dependent nutrient uptake [ J ]. Mol Biol Cell,2002 , 13 (7) : 2276-2288.
  • 4West KA, Brognard J, Clark AS, et al. Rapid Akt activation by nicotine and a tobacco carcinogen modulates the phenotype of normal human airway epithelial cells [ J]. J Clin Invest, 2003,111(1) :81-90.
  • 5Nam SY, Jung GA, Hur GC, et al. Upregulation of FLIP(S) by Akt, a possible inhibition mechanism of TRAIL-induced apoptosis in human gastric cancers [ J ]. Cancer Sci, 2003,94 ( 12 ) : 1066-1073.
  • 6Gagnon V, St-Germain ME, Parent S, et al. Akt activity in endometrial cancer cells: regulation of cell survival through cIAP-1 [ J].Int J Oncol, 2003 ,23(3) :803-810.
  • 7Dan HC, Sun M, Kaneko S, et al. Akt Phosphorylation and Stabilization of X-linked Inhibitor of Apoptosis Protein (XIAP) [ J ]. J Biol Chem, 2004,279(7) :5405-5412.
  • 8Zhang D, Brodt P. Type 1 insulin-like growth factor regulates MT1-MMP synthesis and tumor invasion via PI3-kinase/Akt signaling[J]. Oncogene, 2003,22(7) :974-982.
  • 9Qian Y, Corum L, Meng Q, et al. PI3K induced actin filament remodeling through Akt and p70S6K1 : implication of essential role in cell migration [ J ]. Am J Physiol Cell Physiol, 2004,286 ( 1 ) :C153-C163.
  • 10Kim D, Kim S, Koh H, et al. Akt/PKB promotes cancer cell invasion via increased motility and metalloproteinase production [ J ].FASEB J, 2001,15 ( 11 ) : 1953-1962.

共引文献37

同被引文献13

  • 1张英杰,刘仁光.大鼠急性心肌缺血早期心电图QRS波群和ST段改变[J].中国心血管病研究,2005,3(6):456-458. 被引量：23
  • 2解丽君,张建新,李兰芳.异丙酚对大鼠离体心脏缺血再灌注损伤的保护作用[J].中华麻醉学杂志,2005,25(9):667-670. 被引量：11
  • 3Gujral JS, Bucci TJ, Farhood A, et al. Mechanism of cell death during warm hepatic ischernia-reperfusion in rats: apoptosis or necrosis[ J ]. Hepatology,2001,33 (2) :397 - 405.
  • 4Kohli V,Selzner M,Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemia-reperfusion injury in the rat liver[J]. Transplantation, 1999,67(8):1099- 1105.
  • 5Bardales RH, Xie SS, Schaefer RF, et al. Apoptosis is a major pathway responsible for the resolution of type Ⅱ pneumocytes in acute lung injury[J ]. Am J Pathol, 1996,149 : 845 - 852.
  • 6Murphy PG, Myers DS, Davies MJ, et al. The antioxidant potential of propofol ( 2,6-diisopropylphenol ) [ J ]. Br J Anaesth, 1992, 68(6):613-618.
  • 7Thimmulappa RK, Lee I-I, Rangasamy T, et al. Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis[J] . J Clin Invest, 2006, 116(4) : 994-995.
  • 8Xu JJ, Wang YL. Propofol attenuation of hydrogen peroxide-mediated oxidative stress and apoptosis in cultured cardiomyocytes involves haeme oxygenase- 1 [J]. Eur J Anaesthesiol, 2008, 25 (5) : 395-402.
  • 9Anwar AA, Li FY, Leake DS, et al. Induction of hemeoxygenase 1 by moderately oxidized low-density lipoproteins in human vascular smooth muscle cells: role of mitogen-activated protein kinases and Nrf2[J] . Free Radic Biol Med, 2005, 39 (2) : 227-236.
  • 10Motohashi H, Yamamoto M. Nrf2-Keapl defines a physiologically important stress response mechanism[ J]. Trends Mol Med, 2004, 10(11) : 549-557.

引证文献2

二级引证文献2

山西医科大学学报
2007年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部