摘要
[目的]采用高浓度胰岛素体外诱导培养法建立人肝细胞IR模型,为研究IR的发生发展机制及筛选改善IR的药物提供一种简便可靠的IR细胞模型。[方法]以人肝细胞系L02为研究对象,在含5×10-7 mol/L人胰岛素的培养液培养16 h,未用高浓度胰岛素培养者为对照组。之后两组分别加入0、1、10、50、100、200 ng/ml人胰岛素。采用葡萄糖氧化酶-过氧化物酶法(GOD-POD)法检测培养液中残存葡萄糖含量。[结果]在各浓度的胰岛素刺激下,高胰岛素诱导组培养液中残存葡萄糖含量均显著高于对照细胞(P﹤0.01)。[结论]用含5×10-7 mol/L胰岛素的培养液培养16 h的L02细胞产生胰岛素抵抗,作为IR肝细胞模型可广泛用于胰岛素抵抗的研究。
[Objective]To establish an convenient and reliable insulin-resistant cell model of human liver cell with high concentration insulin,which can be used for the study of insulin-resistant mechanisms and the screening of medicine.[Methods]L02 cells in experimental group were incubated in culture solution containing 5×10-7 mol/L insulin for 16 hours,while L02 cells in control group were incubated without insulin.Then 0 ng/ml、1 ng/ml、10 ng/ml、50 ng/ml、100 ng/ml、200 ng/ml insulin separately were added in culture solution of two groups.The content of glucose remained in L02 cells culture solution was measured by the method of glucose oxidizes peroxides（GOD-POD）.[Results]The content of glucose remained in culture solution added with high concentration of insulin in experimental group was notably higher than that in control group（P﹤0.01）.[Conclusions]The L02 cells which are incubated in culture solution with high concentration of insulin（5×10-7 mol/L）have insulin-resistance.The IR liver cell model can be widely used for the study of insulin resistance.
出处
《现代预防医学》
CAS
北大核心
2007年第22期4233-4234,4236,共3页
Modern Preventive Medicine
基金
重庆医科大学创新基金(CX0528)
