摘要
为了筛选蚬壳花椒组培苗增殖的最优培养方案,对影响组培苗增殖的因素进行了单因素试验.结果显示,以MS为基本培养基,添加0.5～1.0mg/L6-BA,0.05～0.1mg/LNAA和使用添加物D对增殖影响显著,但不同浓度差异不显著.对各单因素进行L9(34)正交试验,结果表明,MS+0.5mg/L6-BA+0.05mg/LNAA+添加物D1.0mg/L为蚬壳花椒增殖的最适培养基,增殖系数可达到4.
To screen on the optimal cultural scheme affecting the induction of Zanthoxylum dissitum Hemsl, the experiment was implemented by two steps. At first, with the single factor method carded out with MS as the optimal basal medium, the optimal cytokinin was 6-BA with concentration 0.5 - 1.0 mg/L; with NAA as the optimal auxin the concentration was 0.05 -0.1 mg/L; no apparent effect of concentration of D was detected. The second, a scheme of orthogonal test, which includes 3 factors and 3 levels, was designed. The results shows： MS ＋6-BA 0.5 mg/L ＋NAA 0.05 mg/L ＋D 1.0 mg/L was the optimal cultural scheme, and the multiplication quotiety can reach 4.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第5期552-555,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
株洲市千金药业股份有限公司项目(102)
中南林业科技大学青年基金项目(7001)
关键词
蚬壳花椒
培养基
增殖
正交试验
Zanthoxylum dissitum Hemsl
culture medium
proliferation
orthogonal test
