摘要
目的建立高效液相色谱法测定盐酸氨溴索注射液的含量的方法。方法色谱柱Kromasil C18柱（150 mm×4.6 mm,5μm）,流动相为乙腈-0.05 mol·L^-1磷酸二氢钾（含0.2%三乙胺,用磷酸调pH值=3.5）（25∶75）,检测波长为245 nm。结果盐酸氨溴索在30～180 mg·L^-1范围内线性关系良好,其回归方程为A=29128C-8748.4,r=0.9998,平均回收率为99.82%（n=5）。结论该法准确,快速,回收率高,适用于盐酸氨溴索注射液的含量测定。
Aim To establish a HPLC method for content determination of ambroxol HCl for injection.Method A Kromasil C18 column（150 mm×4.6 mm,5 μm）was used with acetonitrile-0.05 mol·L^-1 postassium phosphate monobasic solution（mixed 0.2% trielhylamine and adjusted to pH 3.5 with phosphoric acid）（25∶ 75）as mobile phase and a UV detector at 248 nm.Result Ambroxol hydrochloride had a linear range within 30～180 mg·L^-1（r=0.999 8）,the average recovery（n =5） was 99.82%,and RSD was 0.5%.Conslusion A rapid,accurate method for determination of ambroxol HCl was established.
出处
《安徽医药》
CAS
2007年第11期997-998,共2页
Anhui Medical and Pharmaceutical Journal
