高糖对体外培养的人视网膜色素上皮细胞HSP47表达的影响被引量：6

Up-regulation of HSP47 by increased glucose concentration in cultured human retinal pigment epithelial cells

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摘要目的探讨高浓度葡萄糖对体外培养的人视网膜色素上皮(RPE)细胞热休克蛋白47(HSP47)表达的影响。方法RPE细胞分别在5.56mmol/L葡萄糖(对照组)以及25mmol/L葡萄糖(高糖组)的培养条件下,通过RT-PCR检测HSP47mRNA的表达,使用Western blot检测其蛋白水平。结果高糖条件下,HSP47mRNA表达水平显著增加(P<0.05),蛋白水平亦显著增加(P<0.05)。结论HSP47作为一种胶原特异的分子伴侣,在高糖条件下RPE细胞内表达的增加可能是导致增生型糖尿病视网膜病变(PDR)发展的重要因素之一。 Objectives One of characteristics of proliferative diabetic retinopathy （PDR） is the accumulation of extracellular matrix molecules, notably collagen. However, the underlying molecular mechanism responsible for excessive deposition of collagen in fibrotic lesions are not fully understood. Type I to V collagens have been shown to bind to a 47 000 heat shock protein （ HSP47）. Accumulation of HSP47 plays a specific role as a molecular chaperone in the processing of procollagen molecules. This experiment was performed to explore the effect of high concentration glucose on the production of HSP47 in cultured human retinal pigment epithelial （RPE） cells. Methods Human eyeballs were obtained from donors within 8 hours after death. None of donor had any known ocular disease. RPE cells were primary cultured in DMEM containing 10% new-born calf serum and 5.56 mmol/L glucose. Cells from generation 3 and 5 were used for experiments and were respectively cultured in DMEM containing 10% new-born calf serum and 5.56 mmol/L glucose （control group） and 25 mmol/L glucose （high-glucose group）. RT-PCR was used to examine the expression of HSP47 mRNA,and Western blot analysis was used to measure the levels of HSP47 protein. Results The expressions of HSP47 mRNA and HSP47 protein synthesis in human RPE cells in high glucose group were 1.27±0. 18 （Ansa47/Aβ_aotln and 712.92±74.17 ） , showing a obvious up-regulation compared with control group （0. 81 ±0. 14 and 557.87±62.35）（t =5.35,t =4.23,P 〈0.05）. Conclusion As a collagen-specific molecular chaperone, HSP47 plays a specific role in the development of PDR.

作者吕明良李敏夏辉曾水清曾爱萍

机构地区广西壮族自治区人民医院眼科华中科技大学同济医学院附属协和医院眼科

出处《眼科研究》 CSCD 北大核心 2007年第11期858-860,共3页 Chinese Ophthalmic Research

关键词热休克蛋白47 人视网膜色素上皮细胞葡萄糖 heat shock protein 47 human retinal pigment epithelial cell glucose

分类号 R774.1 [医药卫生—眼科]

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