摘要
目的分析栽培与野生肉苁蓉Cistanche deserticola种质资源多样性。方法对58个种植基地和野生肉苁蓉单株进行AFLP分析,利用PopGene32软件分析群体的遗传多样性。结果栽培肉苁蓉多态位点百分率79.16%,野生居群总计多态位点百分率89.53%,4个居群平均Nei's基因多样性指数为0.1938,Shannon's多态性信息指数为0.3004,基因分化系数Gst为0.0979。结论栽培与野生肉苁蓉遗传多样性均较高,遗传分化很小。说明现阶段该物种的种内丰度较高,其濒危原因不在于此。因此,野生抚育和人工栽培是保护肉苁蓉资源并实现可持续利用的根本方向。
Objective To determine the genetic diversity of germplasm resource in cultivated and wild Cistanche deserticola. Methods Fifty-eight samples from three populations of cultivated and wild C. deserticola were analyzed by amplified fragment length polymorphism (AFLP) DNA markers, and the gene- tic diversity was evaluated by PopGen32. Results The average percentage of polymorphic loci (PPL) of cultivated C. deserticola is 79.16%. The PPL of wild population is 89.53%. Average Neis gene diversity index (He) from four populations was 0.193 8, Shannons genetic diversity index (I) was 0.300 4, and genetic differentiation index (Gst) was 0.097 9. Conclusion The diversities of cultivated and wild C. deserticola are both higher and theres no differentiation between them. It shows that genetic diversity of inner-species is higher, which is not the reason for endangerment. Therefore, wild nursery and artificial cultivating are the best measures for the conservation and sustainable utilization in C.deserticola.
出处
《中草药》
CAS
CSCD
北大核心
2007年第11期1703-1707,共5页
Chinese Traditional and Herbal Drugs
基金
科技部国家科技攻关项目:濒危药材肉苁蓉种质资源评价研究(2004BA721A36)
关键词
肉苁蓉
种质资源
遗传多样性
AFLP
Cistanche deserticola Y. C. Ma
germplasm resources
genetic diversity
amplified fragment length polymorphism (AFLP)
