摘要
应用聚合酶链反应技术对１０８名无亲缘关系的武汉地区健康汉族人检测了脂蛋白脂肪酶（ＬＰＬ）基因ＨｉｎｄⅢ位点的限制性片段长度多态性，结果显示存在Ｈ＋和Ｈ－两种等位基因，构成Ｈ＋Ｈ＋、Ｈ＋Ｈ－、Ｈ－Ｈ－３种基因型，其中Ｈ＋Ｈ＋和Ｈ＋Ｈ－基因型之间血清甘油三酯（ＴＧ）和高密度脂蛋白胆固醇（ＨＤＬ－Ｃ）水平有显著差异，提示ＬＰＬ基因ＨｉｎｄⅢ位点多态性可能与正常人群中血脂水平的个体差异密切相关。
unrelated normal Chinese of Wuhan area (male 52 and female 56) were collected to detect Hind Ⅲ restrictive fragment length polymorphism (RFLP) of the Lipoproptein Lipase (LPL) gene by polymerase Chain reaction (PCR). We found that the individuals with genotype H +H - had significantly higher high density lipoprotein cholesterol (HDL C) than that of the H +H + genotype. And the H +H - genotype was associated with lower triglycerides (TG) than that of H +H + genotype. The results suggest that the HindⅢ RFLP of LPL gene seems to be involved in the determination of individual differences in plasma lipid and lipoprotein levels. The H - allele was associated with lower TG level and higher HDL C level.
关键词
脂蛋白脂肪酶
基因
多态性
lipoprotein lipase
genes
polymorphism (genetics)
triglycerides
lipoproteins, HDL.
