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两相体系中固定化细胞生物合成L-苯基乙酰基甲醇 被引量:1

Biotransforming L-PAC with immobilized cell in biphasic aqueous/organic phase
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摘要 目的生物合成L-苯基乙酰基甲醇(L-PAC)。方法在10%石油醚/水两相体系中,利用固定化酵母细胞生物合成L-麻黄素的重要中间体L-PAC。结果确定在最佳温度28℃,pH 6.46,活化时间30 min,反应时间10 h,苯甲醛浓度为3 mL/L,添加少量β-环糊精等的条件下,以2%海藻酸钙和10%聚乙烯醇为复合载体,生物合成L-PAC,产量可达8.34 g/L。结论为生物合成L-PAC提供了一种很好的方法。 Purpose To biotransform L-PAC.Methods As an important precurosor of L-ephedrine,L-phenylacetylcarbinol(L-PAC) can be produced by biotransformation with immobilized cell of yeast in 10% petroleum benzine biphasic aqueous/organic phase.Results Under the conditions of pH 6.46,at 28℃,the concentration of benzaldehyde 3 ml/L,2% calcium alginate and 10% PVA as comp vector and the production of L-PAC 8.34 g/L.Conclusion It provides a good method for the biotransformation of L-PAC.
出处 《中国生化药物杂志》 CAS CSCD 2007年第3期166-169,共4页 Chinese Journal of Biochemical Pharmaceutics
关键词 L-苯基乙酰基甲醇 两相体系 固定化细胞 复合载体 L-phenylacetylcarbinol biphasic aqueous/organic phase immobilized cell comp vector
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参考文献10

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