摘要
本实验建立了采用离子排斥色谱法快速测定山西老陈醋中的8种主要有机酸的方法。选用PRP-X300离子排斥色谱柱,确定最佳色谱分离条件为:0.05mmol/LH2SO4为流动相,流速1ml/min,柱温为室温,紫外检测器,检测波长为210nm,进样量5μl。各有机酸的相对标准偏差为0.72%～5.47%,回收率达到95.1%～105%。该方法具有简便快速、选择性好、准确度高的特点。
An ion-exclusion HPLC method for rapid determination of 8 organic acids in Shanxi ageing vinegar was developed in this paper. 8 organic acids were separated by the use of PRP-X300 ion-exclusion column, and the optimal separation conditions are as follows： 0.05mmol/L H2804 as mobile phase, flow rate at 1ml/min, column temperature at room temperature, UV detector at 210 nm, and sample size as 5 μ 1. The RSD is 0.72% ～ 5.47 %, and the recovery rate is 95.1% ～ 105 %. The method has the advantages of rapid and simple operation, good selectivity and high accuracy.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第11期456-459,共4页
Food Science
基金
山西省重大科技攻关项目
