摘要
通过杀菌性能、蛋白质溶出、添加半胱氨酸等实验，并利用生物透射电镜技术，对含铜铁素体抗菌不锈钢对大肠杆菌的杀灭作用机理进行了初步研究。结果表明抗菌不锈钢对大肠杆菌的杀菌率达到99．99％。进一步的研究发现，大肠杆菌与抗菌不锈钢作用后，其细胞壁和细胞膜破裂，细胞膜脂质和含-SH等还原基团酶发生氧化，细胞内出现了明显的内含物溢出现象。随着与抗菌不锈钢作用时间的延长，细菌细胞内的蛋白质漏出量不断增加，最终致使细菌死亡。
Through experiments of the antibacterial property, protein leaking, adding cysteine as well as the bio-transmission electron microscopic technique, the antibacterial mechanism was primarily studied on the Cu-bearing ferritic antibacterial stainless steel against E.coli. The results showed that the antibacterial stainless steel could reach an antibacterial rate up to 99.99% against E.coli. It was found from further study that after the bacteria acted with the antibacterial stainless steel, their cell walls and cell membranes were broken, the enzymes including cell membrane lipids and-SH appeared oxidative, and the inners of the cells showed obvious leaking of contents. With increase of the action time, more proteins were leaked from the cells of the bacteria, leading to the final death of the bacteria.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2007年第A09期3422-3425,共4页
Journal of Functional Materials
基金
国家自然科学基金资助项目（50671101）
关键词
抗菌不锈钢
大肠杆菌
杀菌机理
antibacterial stainless steel
E.coli
antibacterial mechanism
