摘要
目的研究标记率高且稳定的188Re标记胰岛素样生长因子-1类似物(IGF-1A)的方法。方法采用直接标记法,改变Tween-80用量、SnCl2.2H2O浓度、IGF-1A用量、洗脱液体积等标记条件;分别在不同时间点测定标记率;标记物中加入生理盐水或人血清后不同时间点测定放射化学纯度。结果最佳标记方法为:将100μl SnCl2.2H2O(10mg/ml)加入50μl IGF-1A(2 mg/ml);300μl Na3PO4和10μl 0.1%Tween-80混匀后加入50μl 188ReO4-洗脱液;在IGF-1A体系中加入洗脱液体系,室温反应30 min;加入500μl NaH2PO4将pH值调节到7.0左右,标记完成。最高标记率为(94.07±0.32)%,胶体含量为(5.50±1.50)%。室温下放置6 h放射化学纯度为(89.07±0.74)%,加入人血清放置24 h后放射化学纯度为(76.57±9.96)%。结论用直接标记法进行188Re标记,IGF-1A标记率高且稳定性良好。
Objective To establish a stable method for labeling insulin-like growth factor-1 analogue （IGF-1A） with ^188Re. Methods The directly labeling method was adopted. Several labeling conditions were tested, such as the volume of Tween-80, the concentration of SnCl2·2H2O, the amount of IGF-1A, and the volume of raRe perrhenate. The labeling efficiency was determined from 15 min to 8 h after labeling The in vitro stability of ^188Re-IGF-1A was analyzed by using human serum or sodium chloride as challenging agent, and the labeling efficiency was determined from 2 to 24 h after added challenging agent. Results The optimum labeling conditions were 10 μl 0.1% Tween-80, 100 μl SnCl2·2H2O （10 mg/ml）, 50μA IGF-1A （2 mg/ml）, and 50μl raRe perrhenate, incubated 30 min at room temperature. The labeling efficiency of ^188Re-IGF-1A could reach （94.07±0.32）% and the amount of radiocolloid was （5.50±1.50）%. It was （89.07±0.74）% after incubation for 6 h at room temperature, and was （76.57±9.96）% after incubation for 24 h with human serum. Conclusion This method of labeling IGF- 1A with raRe using SnCl2·2H2O is stable and high labeling efficiency can be obtained.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2007年第5期722-725,共4页
Suzhou University Journal of Medical Science
基金
江苏省135医学重点人才资助项目(RC2002035)
苏州市社会发展项目(SSY0617)
