摘要
目的建立红景天颗粒剂中红景天苷的含量测定方法。方法采用AgilentEclipseXDBCl8色谱柱(4.6mm×250mm,5μm),以乙腈-水(V:V=5.0:95.0)为流动相,检测波长为278nm。结果红景天苷在0.68～3.4μg范围内呈良好线性关系,r=0.9997,平均回收率为98.81%,RSD为1.45%(n=5)。结论该方法可靠、准确、专属性强,可有效控制红景天颗粒剂的质量。
Objective To establish a method for Salidroside in Hongjintian Granules. Methods The HPLC was conducted on an Agilent Eclipse XDB C18 column （4.6 ram×250 mm, 5μm） with Acetonitrile： water （V：V=5.0：95.0） as a mobile phase, detection wavelength at 278 rim. Results Salidroside had a good linear relationship within the range of 0.68-3.4 p,g （r=0.9997）. The average recovery was 98.81%, and the RSD was 1.45% （n=5）. Conclusion The method is reliable, accurate and specific. It can be used for the quality control of Hongjintian Granules.
出处
《上海中医药杂志》
北大核心
2007年第11期74-75,共2页
Shanghai Journal of Traditional Chinese Medicine
