摘要
目的：建立小鼠血浆中β-榄香烯的反相高效液相色谱法含量分析方法。方法：小鼠血浆中加入2倍体积的乙腈提取出β-榄香烯用于测定。色谱柱为Hypersil BDS C18（4．6mm×200mm，5μm），流动相为乙腈-水（体积比90：10），检测波长为205nm，流速为1mL／min。结果：血浆中其他组分不影响β-榄香烯的检测，在0．4～24．0mg／L浓度范围内，线性关系良好（Y=160．73X-24．12，r=0．9996），相对回收率〉（95．70±3．07）％。结论：本方法具有简单、快速、准确等特点，适用于测定血浆中β-榄香烯的浓度。
Aim ：To establish a reversed-phase high-performance liquid chromatography method for determination of β- elemene in mouse plasma. Methods ：β-elemene in mouse plasma was extracted by acetonitrile with a volume ratio of 1 ： 2. The detection was carried out on Hypersil BDS C18 （5 μm, 4.6 mm × 200 mm） by using acetonitrile-water（90 ： 10） as the mobile phase with a flow rate of 1 mL/min at 205 nm. Results： β-elemene could be well separated from other components in plasma. The linear range was 0.4 - 24.0 mg/L（ Y = 160.73X - 24.12 ,r = 0. 999 6） , and the average recovery was more than （95.70± 3.07）%. Conclusion： This method provides a simple, rapid, and accurate way in detection of blood concentration of β-elemene.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2007年第6期1171-1172,共2页
Journal of Zhengzhou University(Medical Sciences)
关键词
Β-榄香烯
反相高效液相色谱
血药浓度
小鼠
β-elemene
reversed-phase high-performance liquid chromatography
drug concentration in plasma
mice
