摘要
目的:评价用3,5,3’,4’盐酸四氢唑啉反1-2二苯乙烯(piceatannol,PICE)抑制细胞信号传导与转录活化因子3(STAT3)的激活,对IFN-α在SK-MEL-28细胞系的抗肿瘤作用的影响。方法:用细胞内染色法检测PICE预处理对IFN-α刺激后SK-MEL-28细胞系内磷酸化STAT1(p-STAT1)和磷酸化STAT3(p-STAT3)表达的变化,MTT比色法检测细胞增殖活性,用ELISA法检测细胞培养上清中的血管内皮细胞生长因子(VEGF)。结果:IFN-α刺激细胞后,细胞内的p-STAT1,p-STAT3的表达均有不同程度的升高,且升高比例与IFN-α浓度成正比;PICE很好地抑制了IFN-α诱导p-STAT3的表达,但对IFN-α诱导的p-STAT1的表达没有影响,并且PICE和IFN-α与肿瘤细胞共培养时,比单用IFN-α能更好地抑制肿瘤细胞生长和抑制STAT3诱导的VEGF的分泌。结论:PICE抑制STAT3的激活,能明显增强SK-MEL-28细胞系的抗肿瘤作用。
Objective： To evaluate the effect by inhibiting the activation of STAT3 by piceatannol （HCE） on the antitumor effect of IFN-α in SK- MEL- 28 cell line. Methods： rllae expression of p - STAT1 and p - STAT3 was measured by intracellular stain, the antiproliferative effects was measured by MTT method and the expression of VEGF was tested by ELISA in SK - MEL - 28 cells pre - treated by PICE and IFN-α. Results： After treatment of the cells with IFN-α, the expression of cellular p - STAT1 and p - STAT3 increased in a hnear pattern with the concentration of IFN-α. PICE inhibited the expression of p - STAT3, but not p - STAT1 induced by IFN-α. When the tumor cells were cultured with both PICE and IFN-α, the effect on inhibiting the growth of tumor cells and the secretion of VEGF induced by p - STAT3 was stronger than the effect when cultured with IFN-α alone. Condusion： The activation of STAT3, which can be inhibited by IFN-α significantly increase the antitumor＇ s effect of IFN-α in SK - MEL - 28 cell hne.
出处
《中国麻风皮肤病杂志》
2007年第11期953-955,共3页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金资助课题
项目编号:306671891
