摘要
用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡,建立VE缺乏雏鸡模型。分离全血淋巴细胞并制备细胞膜,检测淋巴细胞膜超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量;采用荧光偏振法检测淋巴细胞膜流动性。结果表明,VE缺乏雏鸡淋巴细胞膜SOD和GSH-Px活力及膜流动性低于对照组,MDA含量高于对照组,组间差异显著或极显著(P<0.05、P<0.01)。说明VE对维持雏鸡淋巴细胞膜功能起重要作用。
The activity of SOD and GSH-Px and the level of MDA of lymphocyte membrane in the modle chicken with Vitamin E （VE）deficiency that produced by using low content of VE feedstuff and supplying unsaturated fatty acid in the diet Were detected by Test Kit;the membrane fluidity of lymphocyte in the same modle chicken was detected through fluorescence polarization. The results showed that activity of SOD and GSH-Px and fluidity of lymphocyte membrane were significantly lower in experimental chicken than control ones（P〈0.05, P〈0.01）; the MDA content were significantly higher than control ones （P〈0.05）. The results indicated that VE plays an important role in maintaining the function of lymphocyte membrane in chicken.
出处
《中国畜牧兽医》
CAS
2007年第11期20-22,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然基金项目(30471279)
黑龙江省博士后科研启动金(LBH-Q05024)
关键词
维生素E
雏鸡
淋巴细胞膜
抗氧化
膜流动性
VE
chicken, lymphocyte membrane
antioxidation
membrane fluidity
