摘要
建立防风通圣丸中黄芩苷的含量测定方法。采用高效液相色谱法,色谱柱为Waters SunFire C18柱,流动相为甲醇-水-磷酸(45∶55∶0.2),流速为1.0mL·min-1,检测波长为315nm。线性范围是0.056～0.504μg(r=0.9994),平均回收率为99.1%(RSD=2.67%,n=6)。该方法简便、准确、重现性好,可作为防风通圣丸中黄芩苷的含量测定方法。
A method of determination of baicaline in Fangfengtongsheng Pill by HPLC. Baicaline was separated on Waters SunFire C18 Column using methanol- water- phosphoric acid （45 ： 55 ： 0. 2） as mobile phase and UV detection wavelength at 315nm. The linear regression for Fangfengtongsheng Pill was over the range of 0. 056-0. 504μg （r=0. 9994） . The average recovery rate was 99.1% （RSD=2. 67%, n= 6）. This method was suitable for the determination of baiealine in Fangfengtongsheng Pills.
出处
《中国药事》
CAS
2007年第11期902-903,共2页
Chinese Pharmaceutical Affairs
