摘要
背景 Histone deacetylase 禁止者( HDACI )被报导了在房间一直不是的胃的癌症上在 trichostatin A ( TSA )的癌症 cells.The 效果导致 apoptosis 很好， characterized.This 学习被瞄准在人的胃的癌症 SGC-7901 cells.Methods 上探索 TSA 的效果和机制房间与 TSA 被对待并且由房间增长试金，西方的污点， TUNEL 试金， isothiocyanate ( FITC )与 Annexin V 结合了的由荧光黄的流动 cytometry 并且 PI 染色分析了，
Background Histone deacetylase inhibitors （HDACIs） have been reported to induce apoptosis in cancer cells. The effects of trichostatin A （TSA） on gastric cancer cells have not been well characterized. This study was aimed to explore the effects and mechanisms of TSA on human gastric cancer SGC-7901 cells. Methods The cells were treated with TSA and analyzed by cell proliferation assay, Western blot, TUNEL assay, flow cytometry by fluorescein isothiocyanate （FITC） conjugated with Annexin V and PI staining, immunofluorescence analysis, analysis of subcellular fractionation, gene chips and real time polymerase chain reaction （PCR）. Results TSA could inhibit cell growth and induced apoptosis in gastric cancer SGC-7901 cells through the regulation of apoptosis-related genes, such as Bcl-2, Bax and survivin. Further study indicated that the pan-caspase inhibitor z-VAD-fmk did not inhibit the apoptosis induced by TSA, and we did not observe the cleavage of poly ADP ribose polymerase （PARP） after TSA treatment too. In addition, apoptosis inducing factor （AIF） and EndoG were found to translocate from mitochondria to nucleus in the immunofluorescence assay and the Western analysis of subcellular fractionation confirmed the result of immunofluorescence assay. Conclusions The apoptosis induced by TSA in gastric cancer SGC-7901 cells involves a caspase-independent pathway.
关键词
胃癌
细胞凋亡
组蛋白脱乙酰酶抑制剂
癌细胞
gastric cancer
histone deacetylase inhibitor
trichostatin A
apoptosis
