摘要
应用高效液相色谱法／二极管阵列检测器同时测定了飞机草中木犀草素、槲皮素和山柰酚的含量。色谱柱HiQ sil C18W柱（4．6mm×25cm，5μm），流动相V（甲醇）：V（水）：V（磷酸）≈50：49．8：0．2，检测波长槲皮素254nm，木犀草素和山柰酚360nm，温度30℃，流速1mL／min，进样量10μL。确定了以超声波提取法制备飞机草分析样品的方法：溶剂为体积分数85％的乙醇，液固比为10：1（mL／g），提取时间为1h。结果表明，3种黄酮在0．01×10^-3－0．10×10^-3g／mL范同内呈现良好线性关系（R^2〉0．9990），平均加样回收率分别为99．6017％、99．0326％和99．4508％，RSD〈2％。该方法操作简便、准确度高，可快速测定飞机草中木犀草素、槲皮素和山柰酚3种物质的含量。
The contents of luteolin, quercetin and kaempferol in Eupatorium Odoratum Linn were determined with RP-HPLC/PAD method. The chromatographic conditions were chromatographic column HiQ sil C18W, detection wavelength 254 nm for quercetin, 360 nm for luteolin and kaempferol, temperature 30℃, mobile phase CH3OH-H20-H3PO4 （50：49.8：0. 2, volume ratio）, 1 mL/min flow rate, 10μL injection volume. Analysis sample was prepared by ultrasonic extraction from Eupatorium Odoratum Linn with the following conditions：85% ethanol solution as solvent, ratio of liquid to solid 10：1 （mL/g） , extraction time 1 h. The results showed good linearity（R^2 〉0.9990） for the three flavonoids over a range of 0.01 × 10^-3-0.10×10^-3 g/mL, and the average recovery yields were 99.6017% , 99.0326%, and 99.4508% , respectively. The relative standard deviation（RSD） was less than 2%. The method is simple and efficient for the determi-nation of luteolin, quercetin and kaempferol from Eupatorium odortum Linn with high accuracy.
出处
《应用化学》
CAS
CSCD
北大核心
2007年第12期1452-1454,共3页
Chinese Journal of Applied Chemistry
基金
教育部新世纪优秀人才支持计划(NCET-04-0316)
黑龙江省自然科学基金项目(ZJN04-03)资助
关键词
飞机草
黄酮
高效液相色谱
超声提取法
Eupatorium odortum Linn,flavonoid, high performance liquid chromatography （ HPLC）, ultrasonicextraction
