摘要
[目的]探讨肝细胞生长因子（HGF）和血管内皮生长因子（VEGF）在子宫内膜异位症（EMs）发病中的意义。[方法]应用双抗体夹心ABC-酶联免疫吸附法（ELISA）检测25例EMs患者和15例正常对照组腹腔液HGF和VEGF的浓度。[结果]①EMs患者腹腔液中HGF和VEGF浓度Ⅰ-Ⅱ期和Ⅲ-Ⅳ期均显著高于对照组（P〈0.01,P〈0.05）;但Ⅰ-Ⅱ期与Ⅲ-Ⅳ期比较差异无显著性（P〉0.05）,且HGF和VEGF浓度与r-AFS评分均无相关性（r=-0.348,P〉0.05;r=-0.323,P〉0.05）。②EMs患者腹腔液中HGF浓度卵泡期与黄体期比较,差异无显著性（P〉0.05）,VEGF浓度卵泡期较黄体期显著升高（P〈0.05）;对照组两因子浓度在卵泡期和黄体期的差异无显著性（P〉0.05）;卵泡期腹腔液HGF和VEGF浓度在EMs组和对照组间比较,差异有显著性（P〈0.01,P〈0.05）,黄体期腹腔液HGF浓度在两组间比较差异无显著性（P〉0.05）,VEGF浓度差异有显著性（P〈0.05）。③EMs患者腹腔液中HGF和VEGF浓度呈明显正相关（r=0.485,P〈0.05）;而对照组两者无相关性（r=0.372,P〉0.05）。[结论]HGF和VEGF可能参与EMs的发生,且两者在EMs的发生发展中可能起协同作用。EMs患者腹腔液HGF浓度可能与月经周期无关,VEGF浓度可能受卵巢激素周期性调节。
[Objective]To evaluate whether these alteration could provide causative and progressive information.[Methods]The concentration of HGF and VEGF was studied by enzyme-linked immunosorbent assay（ELISA） methods in 25 cases of endometriosis and 15 cases of normal control without endometriosis.[Results]①The HGF and VEGF concentrations in peritoneal fluids of both mild and severe endometriosis groups were significantly higher than in controls（P〈0.01,P〈0.05）,while no significant difference was observed between the mild and severe endometriosis groups（P〉0.05）.There were no correlation between the level of HGF or VEGF and degree of endometriosis（P〉0.05）.②The HGF concentration in peritoneal fluids had no significant difference between the proliferative and secretory phase in endometriosis（P〉0.05）,while VEGF concentration in the proliferative phase was significantly higher than that in secretory phase（P〈0.05）.No cyclic variation in HGF and VEGF was seen in the control group（P〉0.05）.③A positive correlation between peritoneal fluid levels of HGF and VEGF was observed in the endometriosis group（P〈0.05）.[Conclusion]The increased HGF and VEGF secretion involves in the pathogenesis of endometriosis,they can promote the ocurrence and progression of the disease together.Increased levels of HGF in patients with endometriosis are not attributed to the cycle phase,while the level of VEGF maybe is adjusted by female＇s hormone.
出处
《医学临床研究》
CAS
2007年第7期1104-1105,1108,共3页
Journal of Clinical Research
