摘要
【目的】探讨葛根素腹腔注射对慢性阻塞性肺疾病(COPD)大鼠模型炎症、抗氧化及气道重塑的影响。【方法】采用熏吸香烟加气管内注射脂多糖的方法建立大鼠COPD模型,并采用病证相结合的方法分别建立气虚、阴虚、血瘀证COPD模型,测定大鼠血清TNF-α、ET-1、肺泡盥洗液IL-8、肺组织匀浆SOD以及支气管平滑肌厚度及其变化。【结果】模型组的TNF-α、ET-1、IL-8、SOD与正常组相比有显著差异(P<0.01);治疗组TNF-α、ET-1含量明显低于模型组含量,有显著差异(P<0.05),血瘀治疗组的TNF-α含量明显低于模型组含量,有显著差异(P<0.05),ET-1含量明显低于模型组含量,但无统计学差异(P>0.05)。各治疗组的IL-8含量明显低于模型组含量,有显著差异(P<0.05);SOD含量明显高于模型组,有显著差异(P<0.05)。各治疗组之间的TNF-α、ET-1、IL-8、SOD含量两两对比,都未见明显差异(P>0.05);各治疗组的支气管平滑肌厚度明显小于模型组支气管平滑肌厚度,有显著差异(P<0.05),各治疗组之间的支气管平滑肌厚度两两对比,都未见明显差异(P>0.05)。【结论】葛根素能减少大鼠COPD模型炎症介质的释放,具有抗氧化、抑制气道重塑的作用。
[Objective]To study the effect of puerarin in inflammatory , oxidative stress , remodeling of airway on chronic obstructive pulmonary disease（COPD） in rats. [MethodslFifty spragu-dawley rats were randomly divided into five groups. The levels of tumor necrosis factor-α and ET-1 in serum were measured with ELISA. The level of IL-8 in BALF was measured with ELISA. The activity of SOD in bronchial and lung homogenates was determined by RIA method. Lung tissue section stained by HE was observed to study the morphological alternations, inflammatory cells of the bronchial wall were counted. Thickness of the bronchial smooth muscle layer was measured by image analyzer. [Results]The levels of the puerarin groups TNF-α, ET- 1 and IL-8 were lower than the COPD model group （ P〈0.05）. The activity of SOD in the puerarin groups was significantly increased compared to the model group（ P〈0.05）. The numbers of inflammatory cells in the puerarin groups were significantly decreased compared to the model group（ P〈0.05）. Thickness of the bronchial smooth muscle layer in the puerarin groups was significantly decreased compared to the model group（ P 〈0.05）. [Conclusion]Puerarin has good effect on COPD model rats. It can lighten inflammatory action and oxidative stress and perfect the process of the remodeling of the small airways.
出处
《医学临床研究》
CAS
2007年第8期1249-1251,共3页
Journal of Clinical Research
基金
南京市医学重点科研发展项目(ZKX043)
关键词
气管炎
肺疾病
阻塞性
大鼠
tracheitis
lung diseases,obstructive
