摘要
目的通过向兔气管插管内灌入海水，建立海水淹溺致急性呼吸窘迫综合征模型。方法14只新西兰大白兔被随机平分为C组（对照组，无任何处理）和S组（海水组，用海水灌注）。观察分次适量海水灌入气管插管后症状学、血气分析、血液动力学、血清中肿瘤坏死因子（TNF—α）和白细胞介素-6（IL-6）的变化。2h后给每组试验兔拍摄肺CT片，并观察其组织病理学变化。结果与C组相比，S组的O2和CO2指数显著降低（P〈0．01），呼吸动力学和血液动力学的情况明显恶化。TNF—α和IL-6增高，这一结果提示肺损伤发生于兔被海水灌注之后。另外，还发现模型兔有明显的肺水肿、肺泡隔断裂和炎性渗出。结论成功复制了既符合海水淹溺的实际情况，又符合国内外急性呼吸窘迫综合征诊断标准的海水淹溺致急性肺损伤动物模型。
Objective To establish an acute respiratory distress syndrome model by perfusing with seawater into tracheal intubation of rabbits. Methods Fourteen New Zealand rabbits were randomly and equally divided into C group （ control group： no any treatment ） and S group （ seawater group ： seawater perfusing treatment）. The changes of semeiology, blood gas analysis, pneodynamic, hemodynamics and tumor necrosis factor （TNF-α） as well as interleukin 6 （IL-6） from serum were recorded whenever quantity sufficient seawater were infused into lungs of rabbits. After 2 hours, all rabbits in each group were taken lung computer tomography （CT） examination and their changes of histopathology were observed. Results Compared with C group, PaO2 and PaCO2 in S group were significantly decreased （ P 〈 0. 01 ）. The conditions of pneodynamics and hemodynamics were ameliorated. The results of increased TNFα and IL-6 implied that lung injury were occurred after perfused with seawater in rabbits. Furthermore, obvious edema, alveolar atrophy and inflammatory infiltration were found in the model. Conclusions An acute respiratory distress syndrome model by perfusing with quantity sufficient seawater into rabbits has been successfully established.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2007年第5期257-260,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
海后司令部科研计划课题（06-3305）
关键词
海水
急性呼吸窘迫综合征
模型
Seawater
Acute respiratory distress syndrome
Model
